PMID- 1370114
OWN - NLM
STAT- MEDLINE
DCOM- 19920123
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 1
DP  - 1992 Jan
TI  - Genotoxicity of 1,3- and 1,6-dinitropyrene: induction of micronuclei in a panel 
      of mammalian test cell lines.
PG  - 11-7
AB  - 1,3-Dinitropyrene (1,3-DNP) and 1,6-dinitropyrene (1,6-DNP) were assessed for 
      their potential to increase the frequencies of micronuclei in a panel of test 
      cell lines consisting of H4IIEC3/G-, 5L, 5L/r-1,3-DNP1, 208F, V79, 
      V79/r-1,6-DNP1, HepG2 and BWI-J cells, which have been partially characterized 
      for their expression of xenobiotic metabolising enzymes. The micronuclei were 
      analyzed for the presence or absence of kinetochores indicating the occurrence of 
      aneuploidy or chromosome breakage, respectively. 1,3-DNP caused a substantial 
      increase in the frequency of micronuclei only in V79 cells. 1,6-DNP was strongly 
      genotoxic in lines H4IIEC3/G-, 208F, V79 and, to a minor degree, in 
      5L/r-1,3-DNP1. It caused the formation of kinetochore-positive as well as 
      kinetochore-negative micronuclei in V79 cells but only of kinetochore-negative 
      micronuclei in H4IIEC3/G- and 208F cells. 1,6-DNP-induced formation of 
      micronuclei was paralleled by the appearance of multinucleated cells. Treatment 
      of V79 cells with 1,3-DNP resulted in the same types of damage as treatment with 
      1,6-DNP, although considerably higher concentrations were required. The results 
      show that 1,6-DNP can be highly genotoxic in mammalian cells, whereas, at least 
      in the panel of test cell lines used, 1,3-DNP possesses only a low genotoxic 
      activity. 1,3-DNP appears to be activated to genotoxic products in V79 cells by 
      the same pathway(s) as 1,6-DNP.
FAU - Roscher, E
AU  - Roscher E
AD  - Institut fur Toxikologie, GSF-Forschungszentrum fur Umwelt und Gesundheit, 
      Neuherberg/Munich, F.R.G.
FAU - Wiebel, F J
AU  - Wiebel FJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 0 (Pyrenes)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - D09D9L0924 (1,3-dinitropyrene)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular
MH  - Cell Line
MH  - Humans
MH  - Liver Neoplasms
MH  - Liver Neoplasms, Experimental
MH  - Micronucleus Tests
MH  - Mutagens/*pharmacology
MH  - Pyrenes/*pharmacology
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 0165-1218(92)90280-D [pii]
AID - 10.1016/0165-1218(92)90280-d [doi]
PST - ppublish
SO  - Mutat Res. 1992 Jan;278(1):11-7. doi: 10.1016/0165-1218(92)90280-d.