PMID- 1370532
OWN - NLM
STAT- MEDLINE
DCOM- 19920214
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 175
IP  - 1
DP  - 1992 Jan 1
TI  - Molecular analysis of the induction of immunoglobulin E synthesis in human B 
      cells by interleukin 4 and engagement of CD40 antigen.
PG  - 289-92
AB  - The molecular events leading to immunoglobulin E (IgE) synthesis in human sIgE- B 
      cells stimulated with interleukin 4 (IL-4) and anti-CD40 monoclonal antibody 
      (mAb) 626.1 were analyzed. Anti-CD40 mAb increased the levels of IL-4-induced 
      germline C epsilon transcripts and induced the production of mature C epsilon 
      mRNA. These effects were dependent on the presence of IL-4. Nested primer PCR 
      revealed deletional switch recombination occurring only in B cell stimulated with 
      both IL-4 and anti-CD40 mAb. DNA sequence analysis of switch fragments showed 
      direct S mu/S epsilon joining, without the deletions or duplications within S mu 
      often found in B cells stimulated with IL-4 and Epstein-Barr virus. Analysis of 
      the switch junction map sites showed "hot spots" for recombination within S mu, 
      but not within S epsilon. These findings indicate that IL-4 provides a signal to 
      B cells to induce germline C epsilon transcription and concurrent CD40 engagement 
      induces S mu/S epsilon deletional switch recombination, production of mature C 
      epsilon mRNA, and IgE synthesis.
FAU - Shapira, S K
AU  - Shapira SK
AD  - Division of Immunology, Children's Hospital, Boston, Massachusetts 02115.
FAU - Vercelli, D
AU  - Vercelli D
FAU - Jabara, H H
AU  - Jabara HH
FAU - Fu, S M
AU  - Fu SM
FAU - Geha, R S
AU  - Geha RS
LA  - eng
SI  - GENBANK/S40279
SI  - GENBANK/S75511
SI  - GENBANK/S75512
SI  - GENBANK/S75513
SI  - GENBANK/S75514
SI  - GENBANK/S75515
SI  - GENBANK/S75516
SI  - GENBANK/S75517
SI  - GENBANK/X63232
SI  - GENBANK/X63233
GR  - 1 R01 AI-31136/AI/NIAID NIH HHS/United States
GR  - 5P50 CA-39542/CA/NCI NIH HHS/United States
GR  - CA-34546/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD40 Antigens)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antigens, CD/*physiology
MH  - Antigens, Differentiation, B-Lymphocyte/*physiology
MH  - B-Lymphocytes/drug effects/*immunology
MH  - Base Sequence
MH  - CD40 Antigens
MH  - Cloning, Molecular
MH  - Humans
MH  - Immunoglobulin E/biosynthesis/*genetics
MH  - Interleukin-4/*pharmacology
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Restriction Mapping
MH  - Transcription, Genetic
PMC - PMC2119063
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
PMCR- 1992/07/01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PHST- 1992/07/01 00:00 [pmc-release]
AID - 92113471 [pii]
AID - 10.1084/jem.175.1.289 [doi]
PST - ppublish
SO  - J Exp Med. 1992 Jan 1;175(1):289-92. doi: 10.1084/jem.175.1.289.