PMID- 1370681
OWN - NLM
STAT- MEDLINE
DCOM- 19920227
LR  - 20220410
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 175
IP  - 2
DP  - 1992 Feb 1
TI  - Profilins constitute a novel family of functional plant pan-allergens.
PG  - 377-85
AB  - Type I allergy is a major health problem in industrialized countries where up to 
      15% of the population suffer from allergic symptoms (rhinitis, conjunctivitis, 
      and asthma). Previously, we identified a cDNA clone that encoded a birch pollen 
      allergen as profilin. Profilins constitute a ubiquitous family of proteins that 
      control actin polymerization in eukaryotic cells; in particular, profilin 
      participates in the acrosomal reaction of animal sperm cells. Although profilins 
      had been unknown in plants so far, our finding led to the assumption that 
      profilins might have similar functions in pollens during plant fertilization and 
      therefore represent allergenic components in almost all pollens. We show that 
      profilins are prominent allergens that can be isolated from tree pollens (Betula 
      verrucosa, birch), from pollens of grasses (Phleum pratense, timothy grass), and 
      weeds (Artemisia vulgaris, mugwort). About 20% of all pollen allergic patients 
      tested (n = 65) displayed immunoglobulin E (IgE) reactivity to recombinant birch 
      profilin that was expressed in pKK223-3. An IgE inhibition experiment performed 
      with recombinant birch profilin and purified natural profilins from timothy grass 
      and mugwort indicates common IgE epitopes. Moreover, all pollen profilins 
      purified from these far distantly related plant species, and likewise the 
      purified recombinant birch profilin, are able to elicit dose-dependent histamine 
      release via high affinity Fc epsilon receptor of blood basophils from profilin 
      allergic patients. The presence of profilin and possibly related proteins as 
      crossreacting allergenic components in various plants therefore provides an 
      explanation as to why certain allergic patients display type I allergic reactions 
      with pollens and even food from distantly related plants. A functional 
      pan-allergen, like profilin, available as purified recombinant protein, may be a 
      useful diagnostic and probably therapeutic reagent.
FAU - Valenta, R
AU  - Valenta R
AD  - Institute of General and Experimental Pathology, University of Vienna, Austria.
FAU - Duchene, M
AU  - Duchene M
FAU - Ebner, C
AU  - Ebner C
FAU - Valent, P
AU  - Valent P
FAU - Sillaber, C
AU  - Sillaber C
FAU - Deviller, P
AU  - Deviller P
FAU - Ferreira, F
AU  - Ferreira F
FAU - Tejkl, M
AU  - Tejkl M
FAU - Edelmann, H
AU  - Edelmann H
FAU - Kraft, D
AU  - Kraft D
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Allergens)
RN  - 0 (Contractile Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Profilins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/genetics/*immunology/isolation & purification
MH  - Animals
MH  - Chromatography, Affinity
MH  - *Contractile Proteins
MH  - Cross Reactions/immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes/immunology
MH  - Gene Expression
MH  - Histamine Release/immunology
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunoblotting
MH  - Immunoglobulin E/immunology
MH  - Microfilament Proteins/genetics/*immunology/isolation & purification
MH  - Pollen/*immunology
MH  - Profilins
MH  - Rabbits
MH  - Recombinant Proteins/immunology/isolation & purification
MH  - Transfection
PMC - PMC2119109
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
PMCR- 1992/08/01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PHST- 1992/08/01 00:00 [pmc-release]
AID - 92121819 [pii]
AID - 10.1084/jem.175.2.377 [doi]
PST - ppublish
SO  - J Exp Med. 1992 Feb 1;175(2):377-85. doi: 10.1084/jem.175.2.377.