PMID- 1371516
OWN - NLM
STAT- MEDLINE
DCOM- 19920330
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 30
IP  - 2
DP  - 1992 Feb
TI  - Monoclonal antibodies and chemiluminescence immunoassay for detection of the 
      surface protein of human T-cell lymphotropic virus.
PG  - 351-8
AB  - Monoclonal antibodies (MAbs) raised against human T-cell lymphotropic virus type 
      I (HTLV-I) recognized five distinct antigenic domains of viral env gene-encoded 
      proteins. By using recombinant env proteins and synthetic peptides as mapping 
      antigens, it was determined that the most immunogenic region represented a 
      central portion of the retroviral surface protein (domain 2; amino acids 165 to 
      191). However, only a single MAb was able to react strongly with native viral 
      proteins. This antibody (clone 6C2) was directed to an epitope within domain 4 
      (amino acids 210 to 306) of the retroviral env gene and reacted with envelope 
      proteins in both HTLV-I and HTLV-II, as determined by immunoprecipitation, 
      solid-phase binding, and immunoblotting. No reactivity against envelope 
      components of other human retroviruses, including human immunodeficiency virus 
      types 1 and 2, was present. Flow cytometry data demonstrated that MAb 6C2 reacted 
      with cell lines chronically infected with HTLV-I or HTLV-II and also with surface 
      antigens expressed on fresh adult T-cell leukemia cells, following up-regulation 
      with interleukin-2. By a chemiluminescence immunoassay procedure, picogram 
      amounts of viral surface protein could be detected in the unconcentrated 
      supernatants of HTLV-infected cell lines and in diagnostic cultures. Levels of 
      env and gag proteins released by cells into culture supernatants were not 
      directly related to percent expression of cell surface viral-coat proteins. 
      Further, the molar ratio of p19 to gp46 in conditioned media varied from strain 
      to strain, possibly reflecting differences in viral assembly or packaging 
      mechanisms. MAb 6C2 will be of value in characterizing the biochemical and 
      immunological behavior of retroviral env gene proteins and in studying the 
      interaction of HTLV-I and HTLV-II with their receptors.
FAU - Papsidero, L D
AU  - Papsidero LD
AD  - Cellular Products, Inc., Buffalo, New York.
FAU - Dittmer, R P
AU  - Dittmer RP
FAU - Vaickus, L
AU  - Vaickus L
FAU - Poiesz, B J
AU  - Poiesz BJ
LA  - eng
GR  - N01HB67021/HB/NHLBI NIH HHS/United States
GR  - R01HL43602/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Deltaretrovirus Antigens)
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, env)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (HTLV-II Antigens)
RN  - 0 (Peptides)
SB  - IM
GS  - env
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Deltaretrovirus Antigens/*analysis/genetics
MH  - Epitopes/genetics
MH  - Gene Products, env/*analysis/genetics/*immunology
MH  - Genes, env
MH  - HTLV-I Antigens/analysis/genetics
MH  - HTLV-II Antigens/analysis/genetics
MH  - Human T-lymphotropic virus 1/genetics
MH  - Humans
MH  - Immunoassay/*methods
MH  - Luminescent Measurements
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptides/genetics/immunology
PMC - PMC265059
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
PMCR- 1992/02/01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PHST- 1992/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.30.2.351-358.1992 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1992 Feb;30(2):351-8. doi: 10.1128/jcm.30.2.351-358.1992.