PMID- 1371798
OWN - NLM
STAT- MEDLINE
DCOM- 19920409
LR  - 20140729
IS  - 0300-9165 (Print)
IS  - 0300-9165 (Linking)
VI  - 44
IP  - 1
DP  - 1992 Jan
TI  - [A study on the proliferation of the uterine endometrium].
PG  - 21-7
AB  - A proliferative activity study of the endometrium was evaluated in three 
      different parts by means of an immunohistochemical approach and flow cytometry 
      (FCM). The materials were 29 cases of hysterectomies due to uterine myoma. 
      Twenty-one cases were in the proliferative phase, and 8 cases were in the 
      secretory phase. The three parts of the endometrium obtained from the upper, 
      middle and lower part of the uterine body were investigated by an 
      immunohistochemical technique with Ki-67 and anti-DNA polymerase alpha antibody. 
      Single staining with Propidium Iodide (PI) was used for cell cycle analysis in 
      FCM. RESULTS: (1) The rate of positive Ki-67 cells was 25.9 +/- 6.3% in the upper 
      part, 26.0 +/- 3.3% in the middle part, and 25.5 +/- 5.8% in the lower part. And 
      the rate of positive DNA polymerase alpha cell was 27.2 +/- 5.8% in the upper 
      part, 24.0 +/- 2.9% in the middle part, and 24.8 +/- 4.0% in the lower part. The 
      growth potential of each part was not significant. (2) FCM did not detect a 
      significant difference among the three parts of the endometrium in cell cycle 
      analysis. (3) Hematoxylin-eosin staining showed that the upper part was thicker 
      than the lower part and the number of glands in the upper part was more abundant 
      than in the lower part. This difference was significant (p less than 0.01). (4) 
      Ki-67 was recognized even in the early secretory phase. (5) And each segment of 
      the endometrium proliferated evenly, not focussed.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Kokeguchi, S
AU  - Kokeguchi S
AD  - Department of Obstetrics and Gynecology, Okayama University Medical School.
FAU - Tamada, T
AU  - Tamada T
FAU - Kodama, J
AU  - Kodama J
FAU - Hayase, R
AU  - Hayase R
FAU - Sekiba, K
AU  - Sekiba K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Sanka Fujinka Gakkai Zasshi
JT  - Nihon Sanka Fujinka Gakkai zasshi
JID - 7505749
RN  - 0 (Antibodies, Monoclonal)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cell Division
MH  - Culture Techniques
MH  - Endometrium/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Staining and Labeling
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Sanka Fujinka Gakkai Zasshi. 1992 Jan;44(1):21-7.