PMID- 1372138
OWN - NLM
STAT- MEDLINE
DCOM- 19920415
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 187
IP  - 2
DP  - 1992 Apr
TI  - Replication of adeno-associated virus type 2 in human lymphocytic cells and 
      interaction with HIV-1.
PG  - 453-63
AB  - Adeno-associated virus (AAV) is a nonpathogenic parvovirus which normally 
      requires helper adenovirus or herpes-virus for replication. We examined the 
      growth of AAV type 2 in human lymphocytes and its possible interaction with 
      HIV-1. Three B cell lines (CK-B, HS-2, and UC729) and four T cell lines (Molt-4, 
      Jurkat, HUT78, and HUT78+HIV, which is persistently infected with HIV-1) were 
      infected with AAV either in the presence or in the absence of adenovirus. AAV DNA 
      was found in cells of all the lines following incubation with the virus, 
      indicating absorption. AAV DNA replication occurred in most cell lines without 
      particular preference for B or T cells, but only in the presence of helper virus, 
      either adenovirus or Epstein-Barr virus. Expression of AAV proteins was examined 
      by immunoblotting and ELISA, using sera specific for AAV Rep or capsid proteins. 
      The level of AAV protein synthesis correlated with the efficiency of AAV DNA 
      replication, and both varied between cell lines. The yield of infectious AAV was 
      low in most cases, except in one T4 line (Jurkat), where AAV replication and 
      protein synthesis in the presence of adenovirus were very extensive. In HUT78+HIV 
      cells both adenovirus and AAV (in the presence of Ad2) replicated efficiently. 
      The effects of adenovirus plus AAV coinfections on HIV-1 replication, measured by 
      reverse-transcriptase (RT) activity, were mild. Infection with adenovirus or AAV 
      alone resulted in a 60-70% increase in RT activity, while infection with AAV plus 
      adenovirus resulted in a 20% decrease in RT activity. The yield of infectious AAV 
      in this cell line was very low.
FAU - Mendelson, E
AU  - Mendelson E
AD  - Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
FAU - Grossman, Z
AU  - Grossman Z
FAU - Mileguir, F
AU  - Mileguir F
FAU - Rechavi, G
AU  - Rechavi G
FAU - Carter, B J
AU  - Carter BJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Adenoviridae Infections/microbiology
MH  - Cell Division
MH  - Dependovirus/*growth & development
MH  - Gene Expression Regulation, Viral
MH  - HIV Infections/microbiology
MH  - HIV Reverse Transcriptase
MH  - HIV-1/*growth & development
MH  - Humans
MH  - In Vitro Techniques
MH  - RNA-Directed DNA Polymerase/metabolism
MH  - T-Lymphocytes/*microbiology
MH  - Tumor Cells, Cultured
MH  - Viral Proteins/genetics
MH  - *Virus Replication
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1016/0042-6822(92)90447-w [doi]
PST - ppublish
SO  - Virology. 1992 Apr;187(2):453-63. doi: 10.1016/0042-6822(92)90447-w.