PMID- 1372291
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 60
IP  - 4
DP  - 1992 Apr
TI  - Lipooligosaccharides (LOS) of some Haemophilus species mimic human 
      glycosphingolipids, and some LOS are sialylated.
PG  - 1322-8
AB  - The lipooligosaccharides (LOS) of strains of Haemophilus ducreyi, Neisseria 
      gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica contain epitopes 
      that are antigenically and structurally similar to carbohydrates present in human 
      glycosphingolipids. LOS from strains of Haemophilus influenzae and H. influenzae 
      biogroup aegyptius were tested for the binding of monoclonal antibodies (MAbs) 
      that bind to human glycosphingolipids possessing Gal beta 1-4GlcNAc (MAb 3F11) 
      and Gal alpha 1-4Gal beta 1-4Glc (MAb anti-Pk). In solid-phase radioimmunoassays, 
      the LOS of 18 of 19 H. influenzae type b (Hib), 8 of 19 nontypeable H. 
      influenzae, and 10 of 20 H. influenzae biogroup aegyptius strains bound MAb 
      anti-Pk. The LOS of 13 of 19 Hib, 10 of 16 nontypeable H. influenzae, and 2 of 18 
      H. influenzae biogroup aegyptius strains bound MAb 3F11. Neuraminidase treatment 
      of the strains increased the binding of MAb 3F11 by more than twofold in 47% of 
      the H. influenzae strains, suggesting that sialic acid occluded the LOS structure 
      recognized by MAb 3F11. The material released from neuraminidase-treated Hib LOS 
      was confirmed to be sialic acid by high-performance anion-exchange 
      chromatography. A recombinant plasmid containing genes involved in Hib LOS 
      biosynthesis directed the expression (assembly) of the 3F11 epitope in 
      Escherichia coli. These studies demonstrate that H. influenzae and H. influenzae 
      biogroup aegyptius express at least two LOS epitopes that are similar to those 
      present in human glycosphingolipids. Sialic acid was present on the LOS of some 
      H. influenzae strains and prevented the binding of MAb 3F11 to its epitope. The 
      oligosaccharide portion of sialylated LOS may also resemble sialylated 
      oligosaccharides present in human glycosphingolipids (gangliosides).
FAU - Mandrell, R E
AU  - Mandrell RE
AD  - Centre for Immunochemistry, University of California, San Francisco 94143.
FAU - McLaughlin, R
AU  - McLaughlin R
FAU - Aba Kwaik, Y
AU  - Aba Kwaik Y
FAU - Lesse, A
AU  - Lesse A
FAU - Yamasaki, R
AU  - Yamasaki R
FAU - Gibson, B
AU  - Gibson B
FAU - Spinola, S M
AU  - Spinola SM
FAU - Apicella, M A
AU  - Apicella MA
LA  - eng
GR  - AI21620/AI/NIAID NIH HHS/United States
GR  - AI22998/AI/NIAID NIH HHS/United States
GR  - AI24616/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Epitopes)
RN  - 0 (Glycosphingolipids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Sialic Acids)
RN  - 0 (lipid-linked oligosaccharides)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Bacterial/immunology
MH  - Blotting, Western
MH  - Carbohydrate Sequence
MH  - Chromatography, Ion Exchange
MH  - Epitopes
MH  - Glycosphingolipids/*immunology
MH  - Haemophilus influenzae
MH  - Humans
MH  - Lipopolysaccharides/*immunology
MH  - Molecular Sequence Data
MH  - N-Acetylneuraminic Acid
MH  - Neuraminidase/pharmacology
MH  - Plasmids
MH  - Sialic Acids/*analysis
PMC - PMC256999
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
PMCR- 1992/04/01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PHST- 1992/04/01 00:00 [pmc-release]
AID - 10.1128/iai.60.4.1322-1328.1992 [doi]
PST - ppublish
SO  - Infect Immun. 1992 Apr;60(4):1322-8. doi: 10.1128/iai.60.4.1322-1328.1992.