PMID- 1372612
OWN - NLM
STAT- MEDLINE
DCOM- 19920428
LR  - 20190827
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 36
IP  - 1
DP  - 1992 Jan
TI  - Use of bacterial trpE fusion vectors to express and characterize the bovine 
      immunodeficiency-like virus core protein.
PG  - 35-49
AB  - The gag coding region from Bovine Immunodeficiency-like Virus (BIV) was cloned 
      into E. coli and expressed as a bacterial fusion protein. Six different clones 
      spanning various regions of the gag open reading frame were generated. The 
      resulting fusion proteins were expressed at high concentrations and readily 
      purified. A panel of bovine immune sera specifically recognized the recombinant 
      Gag proteins, as did immune sera from animals infected or immunized with 
      lentiviruses related to BIV, such as Equine Infectious Anemia Virus (EIAV) and 
      Human Immunodeficiency Virus (HIV). Analysis of the deletion clones, using the 
      bovine immune sera panel, enabled us to identify at least one major epitope which 
      was specifically recognized by all bovine sera examined. The ease of expression, 
      purification, and specificity of these fusion proteins should enable a thorough 
      study of the epidemiology of BIV infection.
FAU - Atkinson, B
AU  - Atkinson B
AD  - Department of Microbiology, University of Kansas, Lawrence 66045.
FAU - Liu, Z Q
AU  - Liu ZQ
FAU - Wood, C
AU  - Wood C
LA  - eng
GR  - AI-24115/AI/NIAID NIH HHS/United States
GR  - AI-30356/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, gag)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/immunology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Cross Reactions
MH  - Epitopes
MH  - Escherichia coli/genetics
MH  - Female
MH  - Gene Products, gag/*genetics/immunology/metabolism
MH  - Genetic Vectors
MH  - Immunodeficiency Virus, Bovine/*genetics/immunology/metabolism
MH  - Lentivirus/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Recombinant Fusion Proteins/immunology/metabolism
MH  - Restriction Mapping
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 0166-0934(92)90155-7 [pii]
AID - 10.1016/0166-0934(92)90155-7 [doi]
PST - ppublish
SO  - J Virol Methods. 1992 Jan;36(1):35-49. doi: 10.1016/0166-0934(92)90155-7.