PMID- 1372689
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - Simplified mouse peripheral reticulocyte micronucleus test with 
      dimethylnitrosamine.
PG  - 103-7
AB  - The induction of micronuclei by treatment with dimethylnitrosamine was evaluated 
      and compared in peripheral blood and bone marrow cells of male CD-1 mice. 
      Peripheral blood preparations were made on acridine orange (AO)-coated slides and 
      scanned by fluorescence microscopy. A significant increase in micronuclei was 
      observed 24 h after treatment in bone marrow polychromatic erythrocytes, and 
      24-48 h after treatment in peripheral reticulocytes. The peak frequency of 
      micronuclei in peripheral reticulocytes was delayed by about 24 h relative to 
      bone marrow polychromatic erythrocytes. This micronucleus test using peripheral 
      blood was shown to be easy to do and as sensitive as the test using bone marrow 
      cells. From this result, it is concluded that the method with AO-coated slides 
      and peripheral blood is as suitable as bone marrow cells for the micronucleus 
      assay.
FAU - Sato, S
AU  - Sato S
AD  - Toxicology Research Laboratories, Japan Tobacco Inc., Kanagawa.
FAU - Taketomi, M
AU  - Taketomi M
FAU - Morita, T
AU  - Morita T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - M43H21IO8R (Dimethylnitrosamine)
SB  - IM
MH  - Animals
MH  - Bone Marrow/drug effects/pathology
MH  - Dimethylnitrosamine/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90217-N [pii]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):103-7.