PMID- 1372692
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - The micronucleus test of methyl methanesulfonate with mouse peripheral blood 
      reticulocytes using acridine orange-coated slides.
PG  - 117-20
AB  - The usefulness of the micronucleus assay using mouse peripheral blood 
      erythrocytes and acridine orange (AO)-coated slides was evaluated with methyl 
      methanesulfonate (MMS). The micronucleus test was carried out at doses ranging 
      from 20 to 80 mg/kg body weight in CD-1 mice by intraperitoneal injection. 
      Peripheral blood cells were examined from 0 to 72 h after treatment at 12- or 
      24-h intervals. Bone marrow cells from other mice treated with 80 mg/kg MMS were 
      also sampled at the same times. The frequency of micronucleated reticulocytes 
      (MNRETs) increased dose-dependently at every sampling time except 72 h, and the 
      maximum frequency of MNRETs was observed at about 36 h after treatment. 
      Micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow after a dose of 
      80 mg/kg were significantly induced at 12 h to 36 h, and the maximum frequency of 
      MNPCEs was observed at 24 h after treatment. The induction of MNRETs was delayed 
      by about 12 h compared to that of MNPCEs in bone marrow, and the maximum 
      frequencies of MNRETs were lower than those of MNPCEs, but the induction of 
      MNRETs by MMS was significant and dose-dependent. It is concluded, therefore, 
      that bone marrow cells could be replaced by peripheral blood cells as material 
      for the micronucleus assay using AO-coated slides.
FAU - Sugiyama, C
AU  - Sugiyama C
AD  - Shiseido Safety and Analytical Research Center, Yokohama-shi, Japan.
FAU - Miyamae, Y
AU  - Miyamae Y
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Fujino, Y
AU  - Fujino Y
FAU - Mori, M
AU  - Mori M
FAU - Ohara, K
AU  - Ohara K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 50SG953SK6 (Mitomycin)
RN  - AT5C31J09G (Methyl Methanesulfonate)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Bone Marrow/drug effects/pathology
MH  - Dose-Response Relationship, Drug
MH  - Methyl Methanesulfonate/*toxicity
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mitomycin/toxicity
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90220-T [pii]
AID - 10.1016/0165-1218(92)90220-t [doi]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):117-20. doi: 10.1016/0165-1218(92)90220-t.