PMID- 1372695
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - The micronucleus assay with peripheral blood reticulocytes by acridine orange 
      supravital staining with 1-beta-D-arabinofuranosylcytosine.
PG  - 131-7
AB  - Dose-dependent induction of micronuclei with 1-beta-D-arabinofuranosylcytosine 
      (ara-C) was clearly shown in CD-1 mouse peripheral blood reticulocytes (RETs) 
      using an acridine orange (AO) supravital staining method, as well as in the 
      conventional bone marrow assay. The maximum frequencies of micronucleated RETs 
      (MNRETs) in peripheral blood and of micronucleated polychromatic erythrocytes 
      (MNPCEs) in bone marrow were comparable, as shown in two laboratories 
      independently. The maximum frequencies of MNRETs in peripheral blood lagged about 
      24 and 12 h behind those of MNPCEs in bone marrow in experiments with 24- and 
      12-h sampling intervals, respectively. The proportion of each type of RET was 
      examined periodically after treatment with ara-C at doses ranging from 6.25 to 
      50.0 mg/kg. The proportion of type I RETs among total RETs decreased 24 or 48 h 
      after treatment according to the dose level. This suggest that this ratio could 
      be a good indicator of the bone marrow cell toxicity of test chemicals.
FAU - Iwakura, K
AU  - Iwakura K
AD  - Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
FAU - Tamura, H
AU  - Tamura H
FAU - Matsumoto, A
AU  - Matsumoto A
FAU - Ajimi, S
AU  - Ajimi S
FAU - Ogura, S
AU  - Ogura S
FAU - Kakimoto, K
AU  - Kakimoto K
FAU - Matsumoto, T
AU  - Matsumoto T
FAU - Hayashi, M
AU  - Hayashi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 04079A1RDZ (Cytarabine)
RN  - 50SG953SK6 (Mitomycin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cytarabine/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Laboratories/standards
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mitomycin/toxicity
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90223-M [pii]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):131-7.