PMID- 1372698
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - The mouse peripheral blood micronucleus test with 2-acetylaminofluorene using the 
      acridine orange supravital staining method.
PG  - 153-7
AB  - The peripheral blood micronucleus test using the acridine orange (AO) supravital 
      staining method was validated with the potent bone marrow clastogen 
      2-acetylaminofluorene (2-AAF). 2-AAF induced micronuclei in peripheral blood 
      reticulocytes dose-dependently as well as in bone marrow polychromatic 
      erythrocytes. The incidence of micronucleated reticulocytes (MNRETs) peaked 48 h 
      after a single treatment in both CD-1 and BDF1 mice, and the incidence of 
      micronucleated polychromatic erythrocytes (MNPCEs) peaked 24 or 48 h after 
      treatment. The maximum incidences of MNRETs were always higher than those of 
      MNPCEs in both mouse strains treated once. In the double-treatment regime, the 
      maximum incidence of MNRETs was observed at 24 h after the second treatment in 
      each strain. The incidences of MNRETs in BDF1 mice were higher than in CD-1 mice 
      after a single treatment but were comparable after double treatment. These 
      results indicate that the peripheral blood micronucleus test using AO supravital 
      staining is as sensitive as the conventional bone marrow assay. The new staining 
      method can be performed more easily than the original smear method using either 
      bone marrow or peripheral blood cells. Thus, the peripheral blood method using AO 
      supravital staining is a possible alternative to the conventional bone marrow 
      assay.
FAU - Asano, N
AU  - Asano N
AD  - Toxicological Research Division, Nitto Denko Corporation, Osaka, Japan.
FAU - Hagiwara, T
AU  - Hagiwara T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 9M98QLJ2DL (2-Acetylaminofluorene)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - 2-Acetylaminofluorene/*toxicity
MH  - Acridine Orange
MH  - Animals
MH  - Bone Marrow/drug effects/pathology
MH  - Dose-Response Relationship, Drug
MH  - Erythrocytes/drug effects/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
MH  - Species Specificity
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90226-P [pii]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):153-7.