PMID- 1372701
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - Micronucleus induction in mouse peripheral reticulocytes by 
      7,12-dimethylbenz[a]anthracene.
PG  - 169-73
AB  - Micronucleus assays using mouse peripheral blood stained vitally on acridine 
      orange (AO)-coated slides were evaluated at two laboratories with 
      7,12-dimethylbenz[a]anthracene (DMBA) and compared with the standard bone marrow 
      assay. DMBA was administered by single intraperitoneal injection to CD-1 mice at 
      doses ranging from 5 to 80 mg/kg, then 5 microliters of peripheral blood was 
      sampled from a tail vein at 24, 48, 72, 96, and 120 h after treatment. Similar 
      incidences of micronucleated young erythrocytes were observed in peripheral blood 
      reticulocytes and bone marrow polychromatic erythrocytes. The dose response of 
      micronucleated reticulocytes was delayed compared to that of micronucleated 
      polychromatic erythrocytes. The dose-response curves after treatment with DMBA 
      differed depending on the sampling times, which revealed the difficulty of 
      obtaining accurate dose-response relations in the micronucleus assay. The present 
      result demonstrated that the simple and rapid AO supravital staining method is a 
      valuable and easier method for obtaining dose- and time-response data for 
      quantification of micronucleus induction by chemicals.
FAU - Suzuki, T
AU  - Suzuki T
AD  - Division of Genetics and Mutagenesis, National Institute of Hygiene Sciences, 
      Tokyo, Japan.
FAU - Tamai, K
AU  - Tamai K
FAU - Kodama, Y
AU  - Kodama Y
FAU - Asita, A O
AU  - Asita AO
FAU - Matsuoka, A
AU  - Matsuoka A
FAU - Sofuni, T
AU  - Sofuni T
FAU - Kurita, M
AU  - Kurita M
FAU - Ohtsuki, H
AU  - Ohtsuki H
FAU - Hiwatashi, T
AU  - Hiwatashi T
FAU - Hayashi, M
AU  - Hayashi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene/*toxicity
MH  - Animals
MH  - Bone Marrow/drug effects/pathology
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90229-S [pii]
AID - 10.1016/0165-1218(92)90229-s [doi]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):169-73. doi: 10.1016/0165-1218(92)90229-s.