PMID- 1372704
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20151119
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - Micronucleus test with vincristine sulfate and colchicine in peripheral blood 
      reticulocytes of mice using acridine orange supravital staining.
PG  - 187-91
AB  - The induction of micronuclei in mouse peripheral blood reticulocytes (RETs) was 
      studied with the spindle poisons vincristine sulfate (VINC) and colchicine (COL) 
      using acridine orange (AO) supravital staining. Each chemical was studied 
      independently in two laboratories using the same protocol. Blood samples were 
      prepared at 0, 24, 48, and 72 h after a single intraperitoneal treatment with 
      VINC (0.0625, 0.125, and 0.25 mg/kg) or COL (0.25, 0.5, 1.0, and 2.0 mg/kg). Both 
      VINC and COL induced micronucleated RETs (MNRETs) significantly and 
      dose-dependently with a peak at 48 h after treatment. Maximum frequencies of 
      micronucleated polychromatic erythrocytes (MNPCEs) were observed 24 h after 
      treatment with VINC; thus, the transition time from MNPCEs to MNRETs was about 24 
      h. Both spindle poisons gave comparable results in the paired laboratories, 
      indicating that the present AO supravital staining method is highly reproducible.
FAU - Kondo, Y
AU  - Kondo Y
AD  - Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
FAU - Honda, S
AU  - Honda S
FAU - Nakajima, M
AU  - Nakajima M
FAU - Miyahana, K
AU  - Miyahana K
FAU - Hayashi, M
AU  - Hayashi M
FAU - Shinagawa, Y
AU  - Shinagawa Y
FAU - Sato, S
AU  - Sato S
FAU - Inoue, K
AU  - Inoue K
FAU - Nito, S
AU  - Nito S
FAU - Ariyuki, F
AU  - Ariyuki F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 5J49Q6B70F (Vincristine)
RN  - F30N4O6XVV (Acridine Orange)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Colchicine/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mutagens/*toxicity
MH  - Reticulocytes/*drug effects
MH  - Vincristine/*toxicity
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90232-O [pii]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):187-91.