PMID- 1372706
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - Micronucleus evaluation in peripheral blood reticulocytes of mice treated with 
      procarbazine hydrochloride or mitomycin C.
PG  - 197-204
AB  - The usefulness of the acridine orange (AO) supravital staining technique for the 
      mouse peripheral blood reticulocyte micronucleus test was investigated 
      independently by three laboratories using the known clastogens procarbazine 
      hydrochloride (PCZ) and mitomycin C (MMC). In all three laboratories the highest 
      frequencies of micronucleated peripheral blood reticulocytes were observed 48 h 
      after treatment of mice with a single dose of either MMC or PCZ. The animals 
      responded to both chemicals in a dose-dependent manner. Although similar 
      qualitative results were observed, mean micronucleus frequencies induced by a 
      particular dose of a given test chemical did vary quantitatively among the three 
      laboratories. This was most probably due to the use of slightly different scoring 
      criteria by each examiner. This aspect needs special attention. To minimize 
      inter-laboratory variability, therefore, we recommend establishing unequivocal 
      criteria to distinguish the subclass of reticulocytes. These should then be used 
      consistently by all investigators using this method. The most striking advantages 
      of the AO supravital staining technique were the ease of slide preparation, the 
      ease with which reticulocytes and mature erythrocytes could be distinguished by 
      the examiners, and the occurrence of numerous scorable reticulocytes in each 
      microscopic field, which greatly speeded up the manual counting process. The 
      disadvantages of the staining technique were the limited scoring time due to the 
      rapid fading of the fluorescence stain, the degradation of the cells with time, 
      and the frequent need to search for adequate scoring areas within a microscopic 
      field. Based on the data of this study the authors conclude that the AO 
      supravital staining technique is highly suitable for the micronucleus assay in 
      erythrocytic cells of mouse peripheral blood. In addition, we consider the mouse 
      peripheral blood reticulocyte micronucleus test to be a useful tool with which to 
      investigate the clastogenic potential of chemicals in vivo. As pretreatment of 
      mice with Aroclor 1254 markedly increased the effect of PCZ on micronucleus 
      induction, we suggest that the inclusion of inducers of drug metabolizing enzymes 
      in the micronucleus test would be useful for the detection of the clastogenic 
      potential of promutagenic chemicals.
FAU - Romagna, F
AU  - Romagna F
AD  - Sandoz Pharma Ltd., Drug Safety Assessment-Toxicology, Basle, Switzerland.
FAU - Matsumura, H
AU  - Matsumura H
FAU - Watanabe, M
AU  - Watanabe M
FAU - Kato, T
AU  - Kato T
FAU - Shirasu, Y
AU  - Shirasu Y
FAU - Ohmori, K
AU  - Ohmori K
FAU - Yamada, H
AU  - Yamada H
FAU - Sasaki, Y F
AU  - Sasaki YF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 35S93Y190K (Procarbazine)
RN  - 50SG953SK6 (Mitomycin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Laboratories/standards
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/methods
MH  - Mitomycin/*toxicity
MH  - Mutagens/*toxicity
MH  - Procarbazine/*toxicity
MH  - Reticulocytes/*drug effects
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90234-Q [pii]
AID - 10.1016/0165-1218(92)90234-q [doi]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):197-204. doi: 10.1016/0165-1218(92)90234-q.