PMID- 1372708
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - The micronucleus assay using peripheral blood reticulocytes from mitomycin C- and 
      cyclophosphamide-treated rats.
PG  - 209-13
AB  - It used to be believed that the use of rat peripheral blood for the micronucleus 
      assay would be difficult because micronucleated erythrocytes are captured and 
      destroyed by the spleen quickly. We have applied an acridine orange (AO) 
      supravital staining method to rat peripheral blood using AO-coated glass slides. 
      Normal and splenectomized SD rats were treated once with mitomycin C (i.p.) or 
      cyclophosphamide (p.o.), and 5 microliters of blood was collected at intervals 
      from the tail vein between 0 and 72 h after treatment. For comparison, bone 
      marrow cells were smeared conventionally 30 h after treatment. Although the 
      frequencies of spontaneous and chemically induced micronucleated reticulocytes 
      (MNRETs) from normal rats were lower on average in the highest dose group than 
      those of splenectomized rats, the incidence of micronuclei among type I and II 
      reticulocytes in normal rats at 48 h was almost identical to the incidence of 
      RNA-containing erythrocytes with micronucleus in bone marrow. Thus, we suggest 
      that rat peripheral reticulocytes can be used as target cells for the 
      micronucleus assay.
FAU - Hayashi, M
AU  - Hayashi M
AD  - Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, 
      Tokyo, Japan.
FAU - Kodama, Y
AU  - Kodama Y
FAU - Awogi, T
AU  - Awogi T
FAU - Suzuki, T
AU  - Suzuki T
FAU - Asita, A O
AU  - Asita AO
FAU - Sofuni, T
AU  - Sofuni T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 50SG953SK6 (Mitomycin)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cyclophosphamide/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Micronucleus Tests/methods
MH  - Mitomycin/*toxicity
MH  - Mutagens/*toxicity
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reticulocytes/*drug effects
MH  - Splenectomy
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90236-S [pii]
AID - 10.1016/0165-1218(92)90236-s [doi]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):209-13. doi: 10.1016/0165-1218(92)90236-s.