PMID- 1372709
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 278
IP  - 2-3
DP  - 1992 Feb-Mar
TI  - Micronucleus test with mouse peripheral blood erythrocytes by acridine orange 
      supravital staining: the summary report of the 5th collaborative study by 
      CSGMT/JEMS.MMS. The Collaborative Study Group for the Micronucleus Test.
PG  - 83-98
AB  - The main goal of the Collaborative Study Group for the Micronucleus Test (CSGMT) 
      was to validate a new method for the micronucleus test, recently introduced by 
      Hayashi et al. (1990), using mouse peripheral blood cells stained supravitally 
      with acridine orange (AO). The micronucleus tests were performed on CD-1 mice 
      using 23 chemicals with various modes of action. As a rule, one chemical was 
      studied by two participants. Peripheral blood sampled from the same animal was 
      examined 0, 24, 48, and 72 h (or longer) after treatment. The frequencies of 
      micronucleated peripheral reticulocytes (MNRETs) were recorded based on 
      observation of 1000 reticulocytes per mouse. All chemicals induced MNRETs 
      dose-dependently. Interlaboratory differences in the induction of MNRETs were in 
      an acceptable range for most chemicals tested. Although differences were observed 
      with some chemicals, there were no discrepancies in qualitative judgment. Most 
      chemicals gave the greatest response 48 h after treatment, which was less 
      variable than in the bone marrow assay (greatest response, 24-48 h). These 
      results suggest that the peripheral blood assay using the AO supravital staining 
      technique generates reproducible and reliable data to evaluate the clastogenicity 
      of chemicals. This makes the peripheral blood micronucleus assay an attractive 
      alternative to the conventional bone marrow assay.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Alkylating Agents)
RN  - 0 (Amines)
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Mutagens)
RN  - 0 (Polycyclic Compounds)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Alkylating Agents/toxicity
MH  - Amines/toxicity
MH  - Animals
MH  - Antimetabolites, Antineoplastic/toxicity
MH  - Cross-Linking Reagents/toxicity
MH  - Erythrocytes/*drug effects
MH  - Japan
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/*methods
MH  - Mutagens/*toxicity
MH  - Polycyclic Compounds/toxicity
MH  - Reticulocytes/*drug effects
MH  - Societies, Scientific
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0165-1218(92)90215-L [pii]
PST - ppublish
SO  - Mutat Res. 1992 Feb-Mar;278(2-3):83-98.