PMID- 1373193
OWN - NLM
STAT- MEDLINE
DCOM- 19920514
LR  - 20041117
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 36
IP  - 4
DP  - 1992 Apr
TI  - Entero-adherent Escherichia coli is an important diarrhoeagenic agent in infants 
      aged below 6 months in Calcutta, India.
PG  - 264-8
AB  - Escherichia coli adherent to HEp-2 and HeLa cells were isolated from the faeces 
      of 43 (19.7%) of 218 hospitalised infants aged below 6 months with acute 
      diarrhoea. No conventional virulence factors, including enterotoxin 
      production--heat-labile (LT) or heat-stable (ST), the verotoxin (VT) or 
      shiga-like toxin (SLT)--or the invasive phenotype (determined by the Sereny test) 
      could be detected among these isolates. Out of the 43 isolates, 16 (37.2%) were 
      of the known enteropathogenic O:K serogroups--enteropathogenic E. coli (EPEC). 
      The remaining 27 (62.8%) isolates showed different types of adherence to HEp-2 
      and HeLa cells which was diffuse (40.7%), localised (37.0%), or both (22.3%); 
      they were identified as entero-adherent E. coli (EAEC). The EAEC isolates adhered 
      to HEp-2 and HeLa cells in the presence of mannose, lactose, fucose, galactose, 
      and fetuin, indicating that adhesion was not specific for these sugars or 
      glycoprotein. Haemagglutination and the salt aggregation test (SAT) did not 
      correlate with patterns of adherence. The results of this study indicate that 
      LA-EAEC is an important aetiological agent of acute diarrhoea in infants aged 
      below 6 months in Calcutta.
FAU - Ghosh, A R
AU  - Ghosh AR
AD  - National Institute of Cholera and Enteric Diseases, Calcutta, India.
FAU - Nair, G B
AU  - Nair GB
FAU - Naik, T N
AU  - Naik TN
FAU - Paul, M
AU  - Paul M
FAU - Pal, S C
AU  - Pal SC
FAU - Sen, D
AU  - Sen D
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Carbohydrates)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Bacterial Adhesion/drug effects
MH  - Carbohydrates/pharmacology
MH  - Cell Line
MH  - Diarrhea, Infantile/*microbiology
MH  - Escherichia coli/classification/isolation & purification/*pathogenicity
MH  - Escherichia coli Infections/*microbiology
MH  - Feces/microbiology
MH  - Female
MH  - HeLa Cells
MH  - Hemagglutination
MH  - Humans
MH  - India
MH  - Infant
MH  - Male
MH  - Surface Properties
MH  - Virulence
MH  - alpha-Fetoproteins/pharmacology
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1099/00222615-36-4-264 [doi]
PST - ppublish
SO  - J Med Microbiol. 1992 Apr;36(4):264-8. doi: 10.1099/00222615-36-4-264.