PMID- 1373197
OWN - NLM
STAT- MEDLINE
DCOM- 19920514
LR  - 20220228
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 66
IP  - 5
DP  - 1992 May
TI  - Target epitope in the Tax protein of human T-cell leukemia virus type I 
      recognized by class I major histocompatibility complex-restricted cytotoxic T 
      cells.
PG  - 2928-33
AB  - A trans-acting regulatory gene product p40tax (Tax) of human T-cell leukemia 
      virus type I (HTLV-I) is one of the main target antigens recognized by cytotoxic 
      T lymphocytes (CTL) specific for HTLV-I. A CTL epitope within the Tax protein was 
      identified in this report. HTLV-I-specific CD8+ CTL lines established from two 
      HTLV-I carriers with HTLV-I-associated myelopathy or Sjogren syndrome were 
      previously demonstrated to kill predominantly the target cells expressing HTLV-I 
      Tax. The CTL from two patients showed significant levels of cytotoxicity to 
      autologous target cells pulsed with a synthetic peptide of 24 amino acids 
      corresponding to the amino-terminal sequences of the Tax protein. Allogeneic 
      target cells were also sensitized for CTL by this peptide when the target cells 
      have HLA-A2. Tax-specific cytotoxicity, detected as cytolysis of the target cells 
      infected with vaccinia virus-HTLV-I recombinant expressing Tax protein, was 
      almost completely inhibited by competitor cells pulsed with the synthetic 
      peptide. This indicates that a major CTL epitope is present in this peptide. 
      Further analysis using shorter peptides revealed that the core sequence of the 
      CTL epitope was LLFGYPVYV at positions 11 through 19. This sequence can be 
      aligned with the HLA-A2-specific motifs reported recently.
FAU - Kannagi, M
AU  - Kannagi M
AD  - Department of Biodefence and Medical Virology, Kumamoto University Medical 
      School, Japan.
FAU - Shida, H
AU  - Shida H
FAU - Igarashi, H
AU  - Igarashi H
FAU - Kuruma, K
AU  - Kuruma K
FAU - Murai, H
AU  - Murai H
FAU - Aono, Y
AU  - Aono Y
FAU - Maruyama, I
AU  - Maruyama I
FAU - Osame, M
AU  - Osame M
FAU - Hattori, T
AU  - Hattori T
FAU - Inoko, H
AU  - Inoko H
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, tax)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chromosome Mapping
MH  - Epitopes/immunology
MH  - Gene Products, tax/genetics/*immunology
MH  - HLA-A2 Antigen/genetics
MH  - HTLV-I Infections/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Immunity, Cellular/immunology
MH  - Major Histocompatibility Complex/*immunology
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/immunology
MH  - Peptide Fragments/pharmacology
MH  - Sjogren's Syndrome/immunology
MH  - T-Lymphocytes, Cytotoxic/drug effects/*immunology
PMC - PMC241051
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
PMCR- 1992/05/01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
PHST- 1992/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.66.5.2928-2933.1992 [doi]
PST - ppublish
SO  - J Virol. 1992 May;66(5):2928-33. doi: 10.1128/JVI.66.5.2928-2933.1992.