PMID- 1374813
OWN - NLM
STAT- MEDLINE
DCOM- 19920616
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 66
IP  - 6
DP  - 1992 Jun
TI  - Gene for the major antigenic structural protein (p100) of human herpesvirus 6.
PG  - 3918-24
AB  - A human herpesvirus 6 (HHV-6) structural protein of 100 kDa (p100) is the 
      polypeptide most frequently and intensively reactive in immunoblotting analyses 
      with human sera on HHV-6-infected cells or partially purified virions. The gene 
      for p100 was identified by screening a bacteriophage lambda library with 
      monospecific rabbit antisera. The gene codes for a polypeptide of 870 amino acids 
      with a calculated molecular size of 97 kDa. Its amino-terminal third is weakly 
      homologous to the immunogenic basic matrix phosphoprotein pp150 of human 
      cytomegalovirus. Five fragments representing more than 93% of HHV-6 p100 were 
      prokaryotically expressed. The antigenic epitopes of p100 were preliminary mapped 
      by immunoblotting with human sera. They are located within the carboxy-terminal 
      part which is neither homologous nor cross-reactive to pp150 of human 
      cytomegalovirus. Availability of the gene for the immunodominant structural 
      protein should provide tools for studies of pathogenesis by HHV-6.
FAU - Neipel, F
AU  - Neipel F
AD  - Institut fur klinische und molekulare Virologie, Universitat Erlangen-Nurnberg, 
      Germany.
FAU - Ellinger, K
AU  - Ellinger K
FAU - Fleckenstein, B
AU  - Fleckenstein B
LA  - eng
SI  - GENBANK/M87287
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (pp150 protein, Cytomegalovirus)
RN  - 147338-03-2 (p100 protein, Human herpesvirus 6)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Epitopes/*immunology
MH  - Escherichia coli/genetics
MH  - Gene Library
MH  - Herpesvirus 6, Human/*genetics/immunology
MH  - Molecular Sequence Data
MH  - Peptide Fragments/biosynthesis/genetics/immunology
MH  - *Phosphoproteins
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/immunology
MH  - *Viral Matrix Proteins
MH  - Viral Proteins/blood/*genetics/immunology
PMC - PMC241182
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
PMCR- 1992/06/01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.66.6.3918-3924.1992 [doi]
PST - ppublish
SO  - J Virol. 1992 Jun;66(6):3918-24. doi: 10.1128/JVI.66.6.3918-3924.1992.