PMID- 1377930
OWN - NLM
STAT- MEDLINE
DCOM- 19920812
LR  - 20181113
IS  - 0959-9673 (Print)
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Linking)
VI  - 73
IP  - 3
DP  - 1992 Jun
TI  - Characterization of the dengue virus-induced helper cytokine.
PG  - 263-72
AB  - Dengue type 2 virus (DV) induces a subpopulation of T lymphocytes of mouse spleen 
      to secrete a soluble helper cytokine (HF) which enhances the DV-specific IgM 
      antibody plaque forming cells (PFC). The present study undertaken to purify and 
      characterize HF shows that it can be purified by low pressure liquid 
      chromatography (LPLC) using Sephacryl S-200 column. HF consisted of two subunits, 
      having a M(r) of 65-68 kDa on SDS-PAGE, and both had similar activity. The 
      isoelectric point of HF was 6.5. HF-specific antisera (HFAS) raised in mice 
      neutralized the activity of HF in mice, reacted with it in a Western blot assay, 
      and bound HF in an immunosorbent column. HF bound to DV-antigen in an 
      immunosorbent column and enhanced only the DV-specific PFC. HF had no effect on 
      PFC against heterologous antigens such as Japanese encephalitis virus, Coxsackie 
      B4 virus or sheep red blood cells. HF generated in mice of H-2k haplotype, 
      enhanced DV-specific PFC in the same strain of mice but had no effect on that in 
      the H-2d or H-2q haplotype strains of mice. Thus, DV-induced HF with a M(r) of 
      65-68 kDa, antigen-specificity and genetic-restriction differs from most of the 
      similarly acting cytokines but appears similar to the cell-free form of T cell 
      receptor alpha beta dimer.
FAU - Chaturvedi, P
AU  - Chaturvedi P
AD  - Postgraduate Department of Microbiology, KG Medical College, Lucknow, India.
FAU - Mukherjee, R
AU  - Mukherjee R
FAU - Chaturvedi, U C
AU  - Chaturvedi UC
FAU - Mathur, A
AU  - Mathur A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 0 (Cytokines)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chromatography
MH  - Cytokines/*analysis/genetics
MH  - Dengue/immunology
MH  - Dengue Virus/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes/analysis
MH  - Immunoglobulin M/immunology
MH  - Isoelectric Point
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Weight
MH  - T-Lymphocytes, Helper-Inducer/*immunology
PMC - PMC2002344
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
PMCR- 1992/06/01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Int J Exp Pathol. 1992 Jun;73(3):263-72.