PMID- 1377938
OWN - NLM
STAT- MEDLINE
DCOM- 19920813
LR  - 20191021
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 4
IP  - 4
DP  - 1992 Jun
TI  - Detection of amplified DNA sequences in human tumor cell lines by fluorescence in 
      situ hybridization.
PG  - 314-20
AB  - An unambiguous and rapid characterization of amplified DNA sequences in tumor 
      cells is important for the understanding of neoplastic progression. This study 
      was conducted to evaluate the potential of fluorescence in situ hybridization 
      (FISH) to identify such amplified DNA sequences in human tumor cell lines. 
      Applying this technique, we followed the metaphase location and interphase 
      position of amplified DNA sequences corresponding to the SAMK, MYC, and MYCN 
      genes in four cell lines derived from human tumors: two gastric carcinoma lines 
      (KATO III and SNU-16), a neuroblastoma (NUB-7), and a neuroepithelioma (NUB-20) 
      line. In metaphase cells of KATO III, NUB-7, and NUB-20 lines, the amplified 
      regions were clearly visible and easily identified at an intrachromosomal 
      location: in KATO III and NUB-7 at a terminal position and in NUB-20 at an 
      interstitial position. In SNU-16, on the other hand, the amplified SAMK and MYC 
      sequences were identified in extrachromosomal double minute chromosomes (DMs). In 
      this line, the SAMK and MYC sequences were coamplified in the same cells and were 
      colocated on the same DMs. FISH also allowed the identification of amplified DNA 
      sequences in nondividing cells, enabling us to distinguish, at interphase, 
      whether the amplification gave rise to intrachromosomal amplified regions (IARs) 
      or to extrachromosomal DMs. The FISH technique also allowed us to determine at 
      metaphase as well as at interphase the extent of amplification and the size of 
      the IARs.
FAU - Bar-Am, I
AU  - Bar-Am I
AD  - Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, 
      Ramat Aviv, Israel.
FAU - Mor, O
AU  - Mor O
FAU - Yeger, H
AU  - Yeger H
FAU - Shiloh, Y
AU  - Shiloh Y
FAU - Avivi, L
AU  - Avivi L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - DNA Probes/genetics
MH  - DNA, Neoplasm/genetics
MH  - Fluorescence
MH  - Gene Amplification/*genetics
MH  - Humans
MH  - Neoplasms/*genetics
MH  - Nucleic Acid Hybridization/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1002/gcc.2870040407 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1992 Jun;4(4):314-20. doi: 10.1002/gcc.2870040407.