PMID- 1378083
OWN - NLM
STAT- MEDLINE
DCOM- 19920811
LR  - 20131121
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 23
IP  - 3
DP  - 1992 Apr
TI  - Transfer of molecules from glia to axon in the squid may be mediated by glial 
      vesicles.
PG  - 217-30
AB  - Although the transfer of glial proteins into the squid giant axon is well 
      documented, the mechanism of the transfer remains unknown. We examined the 
      possibility that the transfer involved membrane-bound vesicles, by taking 
      advantage of the fact that the fluorescent compound, 3,6-acridinediamine, 
      N,N,N,',N'-tetramethylmonohydride [acridine orange (AO)], rapidly and selectively 
      stains vesicular structures in glial cells surrounding the giant axon. We labeled 
      cleaned axons (1-3 cm long) by incubation for 1 min in filtered seawater (FSW) 
      containing AO. Because the AO was concentrated in glial vesicular organelles, 
      these fluoresced bright orange when the axon was examined by epifluorescence 
      microscopy. To look for vesicle transfer, axoplasm was extruded from such 
      AO-treated axons at various times after labeling. During the initial 15 min, an 
      increasing number of fluorescent vesicles were observed. No further increases 
      were observed between 15 and 60 min post AO. The transfer of the fluorescent 
      vesicles into the axoplasm seemed to be energy dependent, as it was inhibited in 
      axons treated with 2 mM KCN. These results suggest that a special mode of 
      exchange exists between the adaxonal glia and the axon, perhaps involving 
      phagocytosis by the axon of small portions of the glial cells.
FAU - Buchheit, T E
AU  - Buchheit TE
AD  - Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
FAU - Tytell, M
AU  - Tytell M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - F30N4O6XVV (Acridine Orange)
RN  - MQD255M2ZO (Potassium Cyanide)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Axonal Transport/*physiology
MH  - Axons/*metabolism
MH  - Decapodiformes
MH  - Histocytochemistry
MH  - Lasers
MH  - Microscopy, Fluorescence
MH  - Neuroglia/*metabolism/ultrastructure
MH  - Potassium Cyanide/pharmacology
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1002/neu.480230303 [doi]
PST - ppublish
SO  - J Neurobiol. 1992 Apr;23(3):217-30. doi: 10.1002/neu.480230303.