PMID- 1378461
OWN - NLM
STAT- MEDLINE
DCOM- 19920819
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 90
IP  - 1
DP  - 1992 Jul
TI  - Intracellular events in anti-IgE nonreleasing human basophils.
PG  - 92-103
AB  - Histamine release (HR) after activation of human basophils with anti-IgE 
      demonstrates a great variability among different donors. To elucidate the 
      biochemical basis of this phenomenon, we studied the activation of purified 
      basophils from donors that barely demonstrated HR (less than 7%) after 
      stimulation by anti-IgE, although IgE was present on these cells and 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-induced HR was normal. Basophils 
      from anti-IgE "nonreleasers" demonstrated, in contrast to cells from "releasers," 
      hardly any changes in cytosolic-free Ca++ concentration after addition of 
      anti-IgE. However, anti-IgE treatment of basophils from these anti-IgE 
      nonreleasers resulted in at least two intracellular changes. First, a profound 
      inhibition (54.2% +/- 4.2%) of a subsequent fMLP-induced HR was observed. This 
      inhibition caused by anti-IgE was also observed in basophils from anti-IgE 
      releasers treated with wortmannin, an inhibitor of the anti-IgE-induced HR. The 
      time course of the inhibition induced by wortmannin plus anti-IgE on the 
      fMLP-induced HR (half-life maximum, 4 minutes; time of maximum concentration, 10 
      minutes) was comparable to the time course of the anti-IgE-induced HR. Second, 
      anti-IgE did induce homotypic aggregation in these anti-IgE-nonreleasing 
      basophils, like in anti-IgE-releasing basophils. These studies indicate that 
      basophils not responding to anti-IgE with HR or cytosolic-free Ca++ concentration 
      changes do generate intracellular signals that inhibit the subsequent response to 
      a heterologous stimulus and induce homotypic aggregation.
FAU - Knol, E F
AU  - Knol EF
AD  - Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, 
      Amsterdam.
FAU - Mul, F P
AU  - Mul FP
FAU - Kuijpers, T W
AU  - Kuijpers TW
FAU - Verhoeven, A J
AU  - Verhoeven AJ
FAU - Roos, D
AU  - Roos D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Indoles)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - N18RMK75W1 (indo-1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Basophils/chemistry/drug effects/*immunology
MH  - Calcium/analysis
MH  - Cell Separation
MH  - Cytosol/chemistry
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Histamine Release/drug effects/*immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Indoles/pharmacology
MH  - Lymphocyte Activation/drug effects/immunology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Reference Values
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - S0091-6749(06)80015-1 [pii]
AID - 10.1016/s0091-6749(06)80015-1 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 1992 Jul;90(1):92-103. doi: 
      10.1016/s0091-6749(06)80015-1.