PMID- 1378851
OWN - NLM
STAT- MEDLINE
DCOM- 19920826
LR  - 20190827
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 37
IP  - 3
DP  - 1992 Jun
TI  - A new electron microscope positive staining method for viruses in suspension.
PG  - 321-35
AB  - A new procedure for the positive staining of viruses in suspension, the Tokuyasu 
      staining procedure (TSP), was evaluated using a non-enveloped virus, rotavirus; 
      an enveloped virus, rubella virus and two glutaraldehyde-treated enveloped 
      viruses, Human T Cell Lymphotropic Virus Type I (HTLV-I) and Human 
      Immunodeficiency Virus Type 1 (HIV-1) as models. The TSP involves an initial 
      staining of the virus with uranyl acetate (UA) followed by thin embedding in a 
      mixture of UA and polyvinyl alcohol (PVA). Using aqueous UA for the TSP, a 
      combination of positively and negatively stained particles was seen for both 
      rotavirus and rubella virus. With glutaraldehyde-fixed HTLV-I and HIV-1, stain 
      penetration did not occur and only negative staining was observed. The 
      substitution of methanolic UA for aqueous UA in the TSP resulted in only positive 
      staining of rotavirus and rubella virus. The change in procedure also resulted in 
      stain penetration of the glutaraldehyde-fixed HTLV-I and HIV-1 to give positively 
      stained particles. Some novel morphological features of rotavirus and rubella 
      virus structure were observed by the TSP.
FAU - Doultree, J C
AU  - Doultree JC
AD  - Virology Department, Fairfield Hospital, Victoria, Australia.
FAU - Kiernan, R E
AU  - Kiernan RE
FAU - Lee, J Y
AU  - Lee JY
FAU - Bowden, D S
AU  - Bowden DS
FAU - McPhee, D A
AU  - McPhee DA
FAU - Tokuyasu, K T
AU  - Tokuyasu KT
FAU - Marshall, J A
AU  - Marshall JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Organometallic Compounds)
RN  - 0 (Suspensions)
RN  - 12067-99-1 (Phosphotungstic Acid)
RN  - 285PN2K1AO (uranyl acetate)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Glutaral/pharmacology
MH  - HIV-1/drug effects/*ultrastructure
MH  - Human T-lymphotropic virus 1/drug effects/*ultrastructure
MH  - Humans
MH  - Microscopy, Electron
MH  - Organometallic Compounds
MH  - Phosphotungstic Acid
MH  - Rotavirus/*ultrastructure
MH  - Rubella virus/*ultrastructure
MH  - Staining and Labeling/*methods
MH  - Suspensions
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 0166-0934(92)90032-9 [pii]
AID - 10.1016/0166-0934(92)90032-9 [doi]
PST - ppublish
SO  - J Virol Methods. 1992 Jun;37(3):321-35. doi: 10.1016/0166-0934(92)90032-9.