PMID- 1379767
OWN - NLM
STAT- MEDLINE
DCOM- 19920904
LR  - 20190911
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 16
IP  - 2
DP  - 1992
TI  - Polymorphonuclear neutrophil leukocyte function in clinical bovine patients and 
      in cows with or without Staphylococcus aureus mastitis.
PG  - 107-15
AB  - A fluorochrome microassay was used to investigate peripheral blood 
      polymorphonuclear leukocyte (PMNL) function in cattle. Glass-adherent PMNL were 
      reacted with Staphylococcus aureus preincubated in 20% bovine serum for 30, 60 
      and 90 min. Coverslips were stained with acridine orange (AO) followed by crystal 
      violet to quench extracellular bacterial fluorescence. PMNL function was 
      evaluated by counting the number of dead (stained red with AO) and live (stained 
      green with AO) S. aureus contained within 100 PMNL. A phagocytic index was 
      calculated as the average number of bacteria contained within PMNL. The 
      percentage killing of S. aureus was calculated from the average proportion of S. 
      aureus within PMNL that were dead. Six clinically normal Holstein calves, 3-4 
      months of age, were sampled on 6 consecutive days. PMNL phagocytosis and killing 
      did not vary significantly (p greater than 0.05) among repeated samplings per 
      calf. PMNL function increased with increasing time of incubation of PMNL with S. 
      aureus. Means (+/- SD) for percentage killing were 46.7 +/- 13.1, 57.4 +/- 11.6, 
      and 62.1 +/- 9.8% for 30, 60 and 90 min of reaction, respectively. Means (+/- SD) 
      for the phagocytic index were 2.9 +/- 0.8, 3.6 +/- 1.0, and 4.2 +/- 1.1 
      bacteria/PMNL for 30, 60 and 90 min of reaction, respectively. PMNL function was 
      determined in 30 normal cattle of various breeds, age and sex, and these values 
      were pooled to provide normal values for PMNL function. When values for bovine 
      clinical patients (n = 25) with various diagnoses were compared with normal 
      values (defined by the mean +/- 2SD for the 30 normal cattle) for PMNL function, 
      only one patient was observed to exhibit PMNL hypofunction. A cow with 
      disseminated intravascular coagulation in association with peracute coliform 
      mastitis exhibited decreased PMNL killing capacity. Abnormal PMNL function was 
      uncommon in the hospital population studied. Peripheral blood PMNL function was 
      evaluated in lactating Holstein cows with (n = 15) or without (n = 15) chronic 
      subclinical S. aureus mastitis. There was no significant (p greater than 0.05) 
      difference in PMNL function among these cows.
FAU - Anderson, K L
AU  - Anderson KL
AD  - Department of Food Animal and Equine Medicine, College of Veterinary Medicine, 
      North Carolina State University, Raleigh 27606.
FAU - Smith, L A
AU  - Smith LA
FAU - DeGraves, F J
AU  - DeGraves FJ
FAU - Hunt, E
AU  - Hunt E
FAU - Fleming, S A
AU  - Fleming SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - F30N4O6XVV (Acridine Orange)
RN  - J4Z741D6O5 (Gentian Violet)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cattle
MH  - Female
MH  - Gentian Violet
MH  - Male
MH  - Mastitis, Bovine/*immunology
MH  - Neutrophils/*immunology
MH  - Phagocytosis
MH  - Staining and Labeling
MH  - Staphylococcal Infections/immunology/*veterinary
MH  - Staphylococcus aureus/*immunology
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1007/BF01839007 [doi]
PST - ppublish
SO  - Vet Res Commun. 1992;16(2):107-15. doi: 10.1007/BF01839007.