PMID- 1382115
OWN - NLM
STAT- MEDLINE
DCOM- 19921016
LR  - 20071114
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 23
IP  - 2
DP  - 1992 Mar
TI  - Glycoconjugates are stage- and position-specific cell surface molecules in the 
      developing olfactory system, 1: The CC1 immunoreactive glycolipid defines a 
      rostrocaudal gradient in the rat vomeronasal system.
PG  - 120-9
AB  - Primary sensory neurons in the vomeronasal organ (VNO) project axons to the 
      glomeruli of the accessory olfactory bulb (AOB) where they form connections with 
      mitral cell dendrites. We demonstrate here that monoclonal antibodies to specific 
      carbohydrate antigens define stage- and position-specific events during the 
      development of the vomeronasal system (VN). CC1 monoclonal antibodies react with 
      specific N-acetyl galactosamine containing glycolipids. In the embryo, CC1 
      antigens are expressed throughout the VNO and on vomeronasal nerves. Beginning 
      approximately at birth and continuing into adults, CC1 expression is spatially 
      restricted in the VNO to centrally located cell bodies. In the postnatal AOB, CC1 
      is expressed in the nerve layer and glomeruli, but only in the rostral half of 
      the AOB. These data suggest that CC1 antigens may participate in the targeting of 
      axons from centrally located VNO neurons to rostral glomeruli in the AOB. In 
      contrast, CC2 monoclonal antibodies, which recognize complex alpha-galactosyl and 
      alpha-fucosyl glycoproteins and glycolipids, react with all VNO cell bodies and 
      VN nerves from embryonic (E) day 15 to adults. CC2 antibodies do not distinguish 
      rostral from caudal regions of the AOB, nor are the CC2 glycoconjugates 
      developmentally regulated. P-Path monoclonal antibodies, which recognize 
      9-O-acetyl sialic acid, react with cell bodies in the VNO and nerve fibers from 
      E13 to postnatal (P) day 2. P-Path immunoreactivity disappears from the VNO 
      system almost completely by P14, when only a few P-Path reactive nerve fibers can 
      be seen. These studies suggest that specific cell surface glycoconjugates may 
      participate in spatially and temporally selective cell-cell interactions during 
      development and maintenance of vomeronasal connections.
FAU - Schwarting, G A
AU  - Schwarting GA
AD  - Department of Biochemistry, E. K. Shriver Center, Waltham, Massachusetts 02254.
FAU - Deutsch, G
AU  - Deutsch G
FAU - Gattey, D M
AU  - Gattey DM
FAU - Crandall, J E
AU  - Crandall JE
LA  - eng
GR  - HD05515/HD/NICHD NIH HHS/United States
GR  - NS 24386/NS/NINDS NIH HHS/United States
GR  - NS 25580/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carbohydrates)
RN  - 0 (Epitopes)
RN  - 0 (Glycoconjugates)
RN  - 0 (Glycolipids)
RN  - 0 (Membrane Lipids)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Carbohydrates/immunology
MH  - Cells, Cultured
MH  - Epitopes/analysis
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Glycoconjugates/*analysis
MH  - Glycolipids/*analysis
MH  - Male
MH  - Membrane Lipids/*analysis
MH  - Nasal Bone/*chemistry/embryology/growth & development
MH  - Nasal Septum/*chemistry/embryology/growth & development
MH  - Neurons/chemistry
MH  - Olfactory Bulb/*chemistry/embryology/growth & development
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1002/neu.480230203 [doi]
PST - ppublish
SO  - J Neurobiol. 1992 Mar;23(2):120-9. doi: 10.1002/neu.480230203.