PMID- 1382405
OWN - NLM
STAT- MEDLINE
DCOM- 19921022
LR  - 20110727
IS  - 0003-9152 (Print)
IS  - 0003-9152 (Linking)
VI  - 61
IP  - 2
DP  - 1992 Mar 1
TI  - [Relationship between endothelial cells and extracellular matrix: investigation 
      using the model of angiogenesis in vitro].
PG  - 134-49
AB  - Using model of angiogenesis in vitro, the relationship between endothelial cells 
      and extracellular matrix was studied. Endothelial cells of bovine brain 
      microvascular vessels (BBECs), carotid artery (BCECs) and aorta (BAECs) were 
      cultured on type I collagen gel and Matrigel. BBECs make tubular structures and 
      BCECs and BAECs grow and make confluent monolayer on type I collagen gel. BCECs 
      and BAECs make tubular structures when second layer of collagen gel was overlaid. 
      BBECs, BCECs and BAECs rapidly make tubular structures on Matrigel. These 
      morphological changes were not affected by basic fibroblast growth factor. The 
      effect of extracellular matrices on the cell kinetics and morphology of cultured 
      bovine carotid artery endothelial cells was studied. Endothelial cells show a 
      cobble stone appearance on plastic and type I collagen gel. They proliferate and 
      make capillary-like structures on reconstituted gels composed of type I collagen 
      and basement membrane substrata. And that endothelial cells forming 
      capillary-like structures were increased with the increase in the concentration 
      of basement membrane substrata. Transmission electromicroscopic examination study 
      revealed endothelial cells forming capillary-like structures have junctional 
      complexes on type I collagen and its mixture with basement membrane substrata. 
      But endothelial cells on basement membrane substrata have no junctional 
      complexes. These results suggest that BBECs have more potent angiogenic ability 
      than BCECs and BAECs. And that proliferation and morphogenesis of endothelial 
      cells are regulated by extracellular matrices.
FAU - Kaneko, T
AU  - Kaneko T
AD  - Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Geka Hokan
JT  - Nihon geka hokan. Archiv fur japanische Chirurgie
JID - 0421143
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*pathology/ultrastructure
MH  - Extracellular Matrix/*pathology/ultrastructure
MH  - Microscopy, Electron
MH  - Models, Cardiovascular
MH  - Neovascularization, Pathologic/*pathology
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Geka Hokan. 1992 Mar 1;61(2):134-49.