PMID- 1383125
OWN - NLM
STAT- MEDLINE
DCOM- 19921113
LR  - 20041117
IS  - 0272-457X (Print)
IS  - 0272-457X (Linking)
VI  - 11
IP  - 4
DP  - 1992 Aug
TI  - Antibodies raised against peptide fragments of CD16 reacting with membrane and 
      soluble form of CD16. I: Production and characterization.
PG  - 447-59
AB  - Monoclonal antibodies (MAbs) were generated against human CD16 (Fc gamma RIII) by 
      fusion of NS1 myeloma cells with spleen cells from BALB/c mice immunized with 
      synthetic peptide sequences derived from the CD16 genes. After screening, four 
      hybridomas secreting MAbs (2 IgM and 2 IgG) were selected, cloned and 
      characterized for their activity against CD16 by ELISA test, flow cytometry, 
      rosette inhibition and immuno-blotting. MAbs reacted strongly in ELISA with a 
      soluble form of CD16 (sCD16) present in human serum and to a lesser degree with 
      soluble recombinant CD16 (srCD16). The binding characteristics of the four 
      antibodies to the sCD16 were different, implying that the antibodies recognize 
      different CD16 epitopes. FACS analysis of peripheral blood from healthy 
      volunteers demonstrated that these MAbs are highly reactive with membrane CD16 of 
      neutrophils cells (70-95%) and with a subset of lymphocytes (6-14%). These MAbs 
      seem interesting and may lead to the purification of the soluble human CD16 and 
      to the knowledge of its functions, its physiological role and the cellular 
      polymorphism.
FAU - Mouawad, R
AU  - Mouawad R
AD  - Medical Oncology Laboratory, Salpetriere Hospital, Paris, France.
FAU - Visonneau, S
AU  - Visonneau S
FAU - Soubrane, C
AU  - Soubrane C
FAU - Hirel, P H
AU  - Hirel PH
FAU - Autran, B
AU  - Autran B
FAU - Marceau-Suissa, J
AU  - Marceau-Suissa J
FAU - Weil, M
AU  - Weil M
FAU - Khayat, D
AU  - Khayat D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hybridoma
JT  - Hybridoma
JID - 8202424
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/*biosynthesis/isolation & purification
MH  - Antibody Specificity
MH  - Epitopes/chemistry
MH  - Humans
MH  - Hybridomas/immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/*immunology
MH  - Receptors, IgG/chemistry/*immunology
MH  - Solubility
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 10.1089/hyb.1992.11.447 [doi]
PST - ppublish
SO  - Hybridoma. 1992 Aug;11(4):447-59. doi: 10.1089/hyb.1992.11.447.