PMID- 1383379
OWN - NLM
STAT- MEDLINE
DCOM- 19921102
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 176
IP  - 4
DP  - 1992 Oct 1
TI  - Interleukin 8 (IL-8) selectively inhibits immunoglobulin E production induced by 
      IL-4 in human B cells.
PG  - 1227-31
AB  - The effect of interleukin 8 (IL-8) on IL-4-induced immunoglobulin E (IgE) 
      production was studied. IL-4 induced IgE and IgG4 production by tonsillar 
      mononuclear cells (MNC) without affecting IgM, IgG1, IgA, IgG2, or IgG3 
      production. IL-8 inhibited IL-4-induced IgE and IgG4 production, whereas it had 
      no effect on IgM, IgG1, IgA, IgG2, and IgG3 production. The inhibitory effect by 
      IL-8 was specific, since it was blocked by anti-IL-8 mAb, but not by control 
      IgG1. Although interferon gamma (IFN-gamma) also inhibited IgE and IgG4 
      production by MNC stimulated with IL-4, the inhibitory effect of IL-8 was not 
      mediated by IFN-gamma, since the IL-8-induced inhibition could not be blocked by 
      anti-IFN-gamma. Furthermore, anti-IL-8 mAb had no effect on IFN-gamma-induced 
      inhibition. Moreover, addition of IL-5 or IL-6 did not reverse IL-8-induced 
      inhibition of IgE production. In contrast to these observations with MNC, IL-4 
      failed to induce IgE and IgG4 production by purified B cells. However, combined 
      treatment of purified B cells cells with IL-4 and anti-CD40 antibody resulted in 
      IgE but not IgG4 production. IL-8 inhibited this IgE production without affecting 
      IgM, IgG1, IgG2, IgG3, IgG4, or IgA production, whereas IFN-gamma, IFN-alpha, or 
      prostaglandin E2 (PGE2) failed to do so. These results indicate that IL-8 
      antagonizes IL-4-induced IgE production by directly affecting B cells through a 
      specific mechanism that is different from IFN-gamma, IFN-alpha, or PGE2.
FAU - Kimata, H
AU  - Kimata H
AD  - Department of Pediatrics, Kyoto University Hospital, Japan.
FAU - Yoshida, A
AU  - Yoshida A
FAU - Ishioka, C
AU  - Ishioka C
FAU - Lindley, I
AU  - Lindley I
FAU - Mikawa, H
AU  - Mikawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Interleukin-8)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/immunology/physiology
MH  - Antigens, Differentiation, B-Lymphocyte/immunology/physiology
MH  - B-Lymphocytes/drug effects/*immunology
MH  - CD40 Antigens
MH  - Cells, Cultured
MH  - Cytokines/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Immunoglobulin M/biosynthesis
MH  - Interleukin-4/*pharmacology
MH  - Interleukin-8/*pharmacology
MH  - Kinetics
MH  - Monocytes/drug effects/*immunology
MH  - Palatine Tonsil/immunology
MH  - Recombinant Proteins/pharmacology
PMC - PMC2119394
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
PMCR- 1993/04/01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
PHST- 1993/04/01 00:00 [pmc-release]
AID - 93018840 [pii]
AID - 10.1084/jem.176.4.1227 [doi]
PST - ppublish
SO  - J Exp Med. 1992 Oct 1;176(4):1227-31. doi: 10.1084/jem.176.4.1227.