PMID- 1383740
OWN - NLM
STAT- MEDLINE
DCOM- 19921123
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 270
IP  - 2
DP  - 1992 Nov 16
TI  - Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, 
      ellagic acid and indole-3-carbinol in the Salmonella and CHO systems.
PG  - 87-95
AB  - Four naturally occurring compounds, indole-3-carbinol (I3C), apigenin (Api), 
      ellagic acid (EA) and tannic acid (TA), were tested for their inhibitory effects 
      against 1-nitropyrene- (1-NP) or 1,6-dinitropyrene (1,6-DNP)-induced genotoxicity 
      in Salmonella tester strains and Chinese hamster ovary (CHO) cells. Api and TA 
      strongly inhibited the bacterial mutagenesis induced by nitropyrenes, while I3C 
      and EA had little or no effect. For example, in TA98, 0.2 mumole Api resulted in 
      48% and 56% inhibition of the mutagenicity induced by 4 nmole 1-NP and 0.035 
      nmole 1,6-DNP, respectively. With an equal dose, TA caused 46% and 50% reduction 
      of the mutagenicity induced by 1-NP and 1,6-DNP, respectively. As expected, a 
      good correlation was observed between the antimutagenicity of nitropyrenes and 
      their inhibitory effect on nitroreductase activity. This indicated that one of 
      the possible antimutagenic mechanisms of Api or TA was to inactivate the 
      metabolism of nitropyrenes. Two biological end-points, cytotoxicity and 
      sister-chromatid exchange (SCEs), were used to screen the antigenotoxic effects 
      of these compounds in CHO cells. At the sub-cytotoxic dose, I3C, Api and TA all 
      protected against the cytotoxicity induced by 1-NP and 1,6-DNP, but only TA and 
      Api gave a significant reduction of the frequency of SCEs. Moreover, this 
      reduction was found to be highly dose-dependent.
FAU - Kuo, M L
AU  - Kuo ML
AD  - Institute of Toxicology, College of Medicine, National Taiwan University, Taipei.
FAU - Lee, K C
AU  - Lee KC
FAU - Lin, J K
AU  - Lin JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Flavonoids)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Indoles)
RN  - 0 (Oils, Volatile)
RN  - 0 (Pyrenes)
RN  - 19YRN3ZS9P (Ellagic Acid)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - C11E72455F (indole-3-carbinol)
RN  - EC 1.7.- (Nitroreductases)
RN  - TD1665I8Q4 (1-nitropyrene)
SB  - IM
MH  - Animals
MH  - *Antimutagenic Agents
MH  - Antioxidants
MH  - CHO Cells/drug effects
MH  - Chamomile
MH  - Chromosome Aberrations
MH  - Cricetinae
MH  - Cricetulus
MH  - Ellagic Acid/*pharmacology
MH  - Flavonoids/*pharmacology
MH  - Hydrolyzable Tannins/*pharmacology
MH  - Indoles/*pharmacology
MH  - Mutagenicity Tests
MH  - Nitroreductases/metabolism
MH  - Oils, Volatile/*pharmacology
MH  - Plants, Medicinal
MH  - Pyrenes/*toxicity
MH  - Salmonella typhimurium/drug effects
MH  - Sister Chromatid Exchange
EDAT- 1992/11/16 00:00
MHDA- 1992/11/16 00:01
CRDT- 1992/11/16 00:00
PHST- 1992/11/16 00:00 [pubmed]
PHST- 1992/11/16 00:01 [medline]
PHST- 1992/11/16 00:00 [entrez]
AID - 0027-5107(92)90119-M [pii]
AID - 10.1016/0027-5107(92)90119-m [doi]
PST - ppublish
SO  - Mutat Res. 1992 Nov 16;270(2):87-95. doi: 10.1016/0027-5107(92)90119-m.