PMID- 1384395
OWN - NLM
STAT- MEDLINE
DCOM- 19921113
LR  - 20061115
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 234
IP  - 1
DP  - 1992 Sep
TI  - A comparative study on the effects of tumor necrosis factor-alpha (TNF-alpha), 
      human angiogenic factor (h-AF) and basic fibroblast growth factor (bFGF) on the 
      chorioallantoic membrane of the chick embryo.
PG  - 105-15
AB  - The chorioallantoic membrane (CAM) assay is a widely used bioassay for testing 
      angiogenic activities. In the present study we compared the gross and 
      micromorphological effects of three angiogenic factors applied in Elvax carriers 
      on the CAM: Tumor necrosis factor-alpha (TNF-alpha), human angiogenic factor 
      (h-AF), and basic fibroblast growth factor (bFGF). Our question was whether the 
      CAM responds to these factors which have very different actions with a stereotype 
      or with a factor specific reaction. By microangiography and light microscopy, all 
      positive reactions appeared as a spoke-wheel vascular pattern with a bundle of 
      small capillary blood vessels in the center. These vessels were predominantly of 
      a distended type in h-AF and TNF experiments, while narrower capillary vessels 
      followed bFGF application. Chorioallantoic ectoderm and endoderm were thickened 
      by cell accumulation and the mesenchymal stroma of the CAM was edematous and 
      infiltrated with leucocytes in all three reactions. Additionally, bFGF 
      experiments showed areas of densely arranged fibroblasts. Observations in vivo 
      showed chorioallantoic tissue movements as a possible mechanism for the 
      spokewheel vascular pattern. As compared with our results from studies of 
      cytokinetics with bromodeoxyuridine, these current findings indicate that 
      chemotaxis is responsible for the chorioallantoic angiogenic reaction rather than 
      cellular proliferation.
FAU - Olivo, M
AU  - Olivo M
AD  - Department of Anatomy and Embryology, Ruhr-University Bochum, Germany.
FAU - Bhardwaj, R
AU  - Bhardwaj R
FAU - Schulze-Osthoff, K
AU  - Schulze-Osthoff K
FAU - Sorg, C
AU  - Sorg C
FAU - Jacob, H J
AU  - Jacob HJ
FAU - Flamme, I
AU  - Flamme I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Allantois/blood supply/*drug effects
MH  - Angiogenesis Inducing Agents/*pharmacology
MH  - Animals
MH  - Biological Assay
MH  - Chemotaxis/drug effects
MH  - Chick Embryo
MH  - Chorion/blood supply/*drug effects
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Humans
MH  - Neovascularization, Pathologic/chemically induced/pathology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1002/ar.1092340112 [doi]
PST - ppublish
SO  - Anat Rec. 1992 Sep;234(1):105-15. doi: 10.1002/ar.1092340112.