PMID- 1384658
OWN - NLM
STAT- MEDLINE
DCOM- 19921217
LR  - 20191021
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 5
IP  - 1
DP  - 1992 Jul
TI  - Analysis of chromosome 12 aneuploidy in interphase cells from human male germ 
      cell tumors by fluorescence in situ hybridization.
PG  - 21-9
AB  - The i(12p) marker chromosome has been found to be a highly nonrandom chromosome 
      abnormality associated with germ cell tumors (GCTs). We have previously shown 
      that a chromosome 12 centromere specific alpha-satellite DNA probe detects the 
      i(12p) by virtue of differences in the size of the signal originating from the 
      i(12p) and normal chromosome 12 centromeres after fluorescence in situ 
      hybridization (FISH) in metaphase and interphase cells of cultured GCT cell 
      lines. We have now extended this analysis to 72 fresh GCT tumor biopsy specimens. 
      Banded cytogenetic analysis was attempted on each of these tumors, 45 of which 
      were found to be clonally abnormal. Data on i(12p) and chromosome 12 copy number 
      obtained by FISH agreed well with those obtained by cytogenetic analysis. In 
      addition, the FISH method made possible the detection and determination of i(12p) 
      and the chromosome 12 copy number in cases in which conventional cytogenetic 
      analysis was unsuccessful. We found the incidence of i(12p) in seminomas to be 
      low (7%) compared to that in nonseminomas (75%) when tumor biopsy specimens were 
      studied by FISH. Our results show that the FISH technique can be used reliably 
      for detection of the diagnostically and prognostically useful i(12p) marker in 
      GCT tumor biopsy specimens.
FAU - Rodriguez, E
AU  - Rodriguez E
AD  - Laboratory of Cancer Genetics, Sloan-Kettering Institute, New York, New York.
FAU - Mathew, S
AU  - Mathew S
FAU - Mukherjee, A B
AU  - Mukherjee AB
FAU - Reuter, V E
AU  - Reuter VE
FAU - Bosl, G J
AU  - Bosl GJ
FAU - Chaganti, R S
AU  - Chaganti RS
LA  - eng
GR  - CA-05826/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Aneuploidy
MH  - Biomarkers, Tumor
MH  - Centromere/ultrastructure
MH  - Chromosome Deletion
MH  - *Chromosome Inversion
MH  - Chromosomes, Human, Pair 12/*ultrastructure
MH  - DNA, Satellite/analysis
MH  - Dysgerminoma/genetics/pathology
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Neoplasms, Germ Cell and Embryonal/*genetics/pathology
MH  - Nondisjunction, Genetic
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - 10.1002/gcc.2870050104 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1992 Jul;5(1):21-9. doi: 10.1002/gcc.2870050104.