PMID- 1385119
OWN - NLM
STAT- MEDLINE
DCOM- 19921203
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 209
IP  - 2
DP  - 1992 Oct 15
TI  - Characterization of the reverse transcriptase of 1731, a Drosophila melanogaster 
      retrotransposon.
PG  - 523-31
AB  - The nucleotide sequence of 1731, a retrotransposon cloned from the genome of 
      Drosophila melanogaster, reveals a structural similarity with the proviral form 
      of the retroviruses including a pol-like gene containing a putative 
      reverse-transcriptase(RT)-coding sequence. Diverse parts of that sequence were 
      subcloned and expressed in Escherichia coli. It has been demonstrated that the 
      expression of the RT-like sequence, when translated, gives rise to peptides 
      displaying enzyme activity characteristic of a true RT enzyme. In addition, 
      rabbit antisera directed against such recombinant proteins allowed us to detect 
      an immunoreactive protein of around 110 kDa, which was only present in D. 
      melanogaster cell lines, but not in cells derived from Drosophila virilis or 
      Drosophila hydei, whose genomes do not bear the 1731 element. This protein is 
      expected to correspond to a non-processed pol-gene translated product and 
      cosediments with virus-like particles exhibiting RT activity.
FAU - Champion, S
AU  - Champion S
AD  - Institut de Chimie Biologique, Universite de Provence, Marseille, France.
FAU - Maisonhaute, C
AU  - Maisonhaute C
FAU - Kim, M H
AU  - Kim MH
FAU - Best-Belpomme, M
AU  - Best-Belpomme M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
GS  - pol
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Chromatography, DEAE-Cellulose
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Cloning, Molecular
MH  - *DNA Transposable Elements
MH  - DNA-Directed DNA Polymerase/isolation & purification/metabolism
MH  - Drosophila/enzymology/genetics
MH  - Drosophila melanogaster/*enzymology/*genetics
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - RNA-Directed DNA Polymerase/*genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Restriction Mapping
MH  - Retroviridae/enzymology/*genetics
EDAT- 1992/10/15 00:00
MHDA- 1992/10/15 00:01
CRDT- 1992/10/15 00:00
PHST- 1992/10/15 00:00 [pubmed]
PHST- 1992/10/15 00:01 [medline]
PHST- 1992/10/15 00:00 [entrez]
AID - 10.1111/j.1432-1033.1992.tb17316.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1992 Oct 15;209(2):523-31. doi: 
      10.1111/j.1432-1033.1992.tb17316.x.