PMID- 1386873
OWN - NLM
STAT- MEDLINE
DCOM- 19920916
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 176
IP  - 2
DP  - 1992 Aug 1
TI  - Identification of the low affinity receptor for immunoglobulin E on mouse mast 
      cells and macrophages as Fc gamma RII and Fc gamma RIII.
PG  - 469-75
AB  - In addition to their well characterized high affinity immunoglobulin E (IgE) 
      receptors (Fc epsilon RI) mast cells have long been suspected to express 
      undefined Fc receptors capable of binding IgE with low affinity. In this paper, 
      we show that Fc gamma RII and Fc gamma RIII, but not Mac-2, on mouse mast cells 
      and macrophages bind IgE-immune complexes. This binding is efficiently competed 
      by 2.4G2, a monoclonal antibody against the extracellular homologous region of 
      both Fc gamma RII and Fc gamma RIII. Furthermore, IgE-immune complexes bind 
      specifically to Fc gamma RII or Fc gamma RIII transfected into COS-7 cells. The 
      association constants of IgE binding estimated from competition experiments are 
      about 3.1 x 10(5) M-1 for Fc gamma RII, and 4.8 x 10(5) M-1 for Fc gamma RIII. 
      Engagement of Fc gamma RII and Fc gamma RIII with IgE-immune complexes (after 
      blocking access to Fc epsilon RI) or with IgG-immune complexes triggers C57.1 
      mouse mast cells to release serotonin. This release is inhibited by 2.4G2, and at 
      maximum, reaches 30-40% of the intracellular content, about half of the maximal 
      release (60-80%) obtained after Fc epsilon RI engagement. These data demonstrate 
      that mouse Fc gamma RII and Fc gamma RIII are not isotype specific, and that the 
      binding of IgE-immune complexes to these receptors induces cell activation.
FAU - Takizawa, F
AU  - Takizawa F
AD  - Molecular Allergy and Immunology Section, National Institute of Allergy and 
      Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852.
FAU - Adamczewski, M
AU  - Adamczewski M
FAU - Kinet, J P
AU  - Kinet JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, IgG)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigen-Antibody Complex
MH  - Antigens, Differentiation/*analysis
MH  - Antigens, Differentiation, B-Lymphocyte/*analysis/metabolism
MH  - Cell Line
MH  - Immunoglobulin E/*metabolism
MH  - Macrophages/*immunology
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Receptors, Fc/*analysis/antagonists & inhibitors/metabolism
MH  - Receptors, IgE
MH  - Receptors, IgG
MH  - Serotonin/metabolism
MH  - Transfection
PMC - PMC2119311
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
PMCR- 1993/02/01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
PHST- 1993/02/01 00:00 [pmc-release]
AID - 92364549 [pii]
AID - 10.1084/jem.176.2.469 [doi]
PST - ppublish
SO  - J Exp Med. 1992 Aug 1;176(2):469-75. doi: 10.1084/jem.176.2.469.