PMID- 1387671
OWN - NLM
STAT- MEDLINE
DCOM- 19921007
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 149
IP  - 6
DP  - 1992 Sep 15
TI  - Potential role of monocyte chemoattractant protein 1/JE in 
      monocyte/macrophage-dependent IgA immune complex alveolitis in the rat.
PG  - 2147-54
AB  - We have examined the role of monocyte chemoattractant protein 1 (MCP 1) in the 
      pathogenesis of monocyte/macrophage-dependent IgA immune complex alveolitis in 
      the rat. Rat MCP 1 was cloned and expressed in order to facilitate analysis of 
      its function in rat models of human disease. A cDNA library was constructed from 
      rat pulmonary artery endothelial cells stimulated with TNF-alpha. The cDNA 
      library was screened with synthetic oligonucleotide probes based on the recently 
      published rat MCP 1 cDNA sequence. Among numerous MCP 1-positive clones, four 
      full length (approximately 480 bp) cDNA were rescued, amplified by polymerase 
      chain reaction, and ligated into a pJVETLZ baculovirus transfer vector. 
      Spodoptera frugiperda insect cells (Sf-21) infected with baculovirus recombinants 
      (Auto-grapha california nuclear polyhedrosis virus) bearing properly oriented MCP 
      1 cDNA (AcMCP 1) directed the expression of unique peptides of 18, 21, and 23 
      kDa. Treatment of AcMCP 1-infected Sf-21 cells with tunicamycin resulted in 
      reduced production of the 21- and 23-kDa proteins and an increase in 16- to 
      18-kDa products, the predicted size range of uncleaved and nonglycosylated rat 
      MCP 1. Denatured and refolded 23-kDa and 21-kDa rat MCP 1 species exhibited 
      dose-dependent monocyte-specific chemotactic activity at concentrations as low as 
      10(-10) M whereas the 18-kDa species exhibited negligible activity. Antibodies 
      that react with the immunoblot, block rat rMCP 1-directed monocyte chemotaxis, 
      and neutralize monocyte-specific chemotactic activity secreted by TNF-stimulated 
      rat endothelial cells were raised in rabbits immunized with the 23-kDa MCP 1 
      species. Intravenous administration of anti-MCP 1 antibodies upon initiation of 
      IgA immune complex lung injury resulted in a marked reduction in lung injury as 
      measured by pulmonary vascular permeability, alveolar hemorrhage, and pulmonary 
      monocyte/macrophage recruitment and pulmonary monocyte/macrophage recruitment. 
      These data suggest that MCP 1 may play an important role in the pathogenesis of 
      monocyte/macrophage-dependent IgA immune complex alveolitis in the rat.
FAU - Jones, M L
AU  - Jones ML
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0602.
FAU - Mulligan, M S
AU  - Mulligan MS
FAU - Flory, C M
AU  - Flory CM
FAU - Ward, P A
AU  - Ward PA
FAU - Warren, J S
AU  - Warren JS
LA  - eng
GR  - 5T32-HL-07517/HL/NHLBI NIH HHS/United States
GR  - HL-40526/HL/NHLBI NIH HHS/United States
GR  - HL-48287/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Proteins)
RN  - 11089-65-9 (Tunicamycin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antigen-Antibody Complex
MH  - Base Sequence
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*physiology
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - Immune Complex Diseases/*immunology
MH  - Immunoglobulin A/immunology
MH  - Immunologic Techniques
MH  - Lung Diseases/*immunology
MH  - Macrophages/*immunology
MH  - Molecular Sequence Data
MH  - Monocytes/*immunology
MH  - Oligodeoxyribonucleotides/chemistry
MH  - Rats
MH  - Recombinant Proteins
MH  - Tunicamycin/pharmacology
EDAT- 1992/09/15 00:00
MHDA- 1992/09/15 00:01
CRDT- 1992/09/15 00:00
PHST- 1992/09/15 00:00 [pubmed]
PHST- 1992/09/15 00:01 [medline]
PHST- 1992/09/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1992 Sep 15;149(6):2147-54.