PMID- 1388095
OWN - NLM
STAT- MEDLINE
DCOM- 19921019
LR  - 20220321
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 70
IP  - 6
DP  - 1992 Sep 18
TI  - Regulation of retinoblastoma protein functions by ectopic expression of human 
      cyclins.
PG  - 993-1006
AB  - The retinoblastoma susceptibility gene (RB) product, the retinoblastoma protein 
      (pRb), functions as a regulator of cell proliferation. Introduction of the RB 
      gene into SAOS-2 osteosarcoma cells, which lack functional pRb, prevents cell 
      cycle progression. Such growth-suppressive functions can be modulated by 
      phosphorylation of pRb, which occurs via cell cycle-regulated kinases. We show 
      that constitutively expressed cyclins A and E can overcome pRb-mediated 
      suppression of proliferation. pRb becomes hyperphosphorylated in cells 
      overexpressing these cyclins, and this phosphorylation is essential for cyclin A- 
      and cyclin E-mediated rescue of pRb-blocked cells. This suggests that G1 and S 
      phase cyclins can act as regulators of pRb function in the cell cycle by 
      promoting pRb phosphorylation.
FAU - Hinds, P W
AU  - Hinds PW
AD  - Whitehead Institute, Cambridge, Massachusetts 02142.
FAU - Mittnacht, S
AU  - Mittnacht S
FAU - Dulic, V
AU  - Dulic V
FAU - Arnold, A
AU  - Arnold A
FAU - Reed, S I
AU  - Reed SI
FAU - Weinberg, R A
AU  - Weinberg RA
LA  - eng
GR  - CA55909/CA/NCI NIH HHS/United States
GR  - GM38328/GM/NIGMS NIH HHS/United States
GR  - GM46006/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Cyclins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Cell Nucleus
MH  - Cyclins/metabolism/*physiology
MH  - G1 Phase/physiology
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Humans
MH  - Mutation
MH  - Phenotype
MH  - Phosphorylation
MH  - Protein Kinases
MH  - Recombinant Proteins
MH  - Retinoblastoma Protein/*physiology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1992/09/18 00:00
MHDA- 1992/09/18 00:01
CRDT- 1992/09/18 00:00
PHST- 1992/09/18 00:00 [pubmed]
PHST- 1992/09/18 00:01 [medline]
PHST- 1992/09/18 00:00 [entrez]
AID - 0092-8674(92)90249-C [pii]
AID - 10.1016/0092-8674(92)90249-c [doi]
PST - ppublish
SO  - Cell. 1992 Sep 18;70(6):993-1006. doi: 10.1016/0092-8674(92)90249-c.