PMID- 1391969
OWN - NLM
STAT- MEDLINE
DCOM- 19921119
LR  - 20131121
IS  - 0916-8478 (Print)
IS  - 0916-8478 (Linking)
VI  - 37
IP  - 2
DP  - 1992 Jun
TI  - Detection of variation in the ribosomal RNA gene clusters by a modified 
      fluorescence in situ hybridization method.
PG  - 139-43
AB  - Physical mapping of genes by fluorescence in situ hybridization (FISH) has become 
      routine using fluorescein isothiocyanate (FITC) for probe detection and propidium 
      iodide (PI) for chromosome staining. We have modified this conventional FISH 
      method in a way that utilizes Texas red (TR) for signal detection and quinacrine 
      mustard (QM) for chromosome banding. Using this Texas red and quinacrine (TRQ) 
      method, we were able to identify individual acrocentric chromosomes with varying 
      degrees of ribosomal RNA gene clusters. Two acrocentric chromosomes were found to 
      carry extremely small number of rRNA gene copies as compared to the other eight 
      counterparts in human diploid lymphoblastoid cell line GM00130B. Thus, the TRQ 
      method allows one to probe for a specific sequence while identifying individual 
      chromosomes and will be powerful for the chromosomal localization of various 
      genes.
FAU - Fukuyama, R
AU  - Fukuyama R
AD  - Department of Molecular Biology, Keio University School of Medicine, Tokyo, 
      Japan.
FAU - Shimizu, N
AU  - Shimizu N
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Hum Genet
JT  - The Japanese journal of human genetics
JID - 9213239
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Xanthenes)
RN  - 82354-19-6 (Texas red)
RN  - H0C805XYDE (Quinacrine)
SB  - IM
MH  - Cell Line
MH  - Diploidy
MH  - Fluorescent Dyes
MH  - *Genetic Variation
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Lymphocytes
MH  - Microscopy, Fluorescence
MH  - *Multigene Family
MH  - Quinacrine
MH  - Xanthenes
MH  - *rRNA Operon
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1007/BF01899735 [doi]
PST - ppublish
SO  - Jpn J Hum Genet. 1992 Jun;37(2):139-43. doi: 10.1007/BF01899735.