PMID- 1395169
OWN - NLM
STAT- MEDLINE
DCOM- 19921106
LR  - 20111117
IS  - 0301-0430 (Print)
IS  - 0301-0430 (Linking)
VI  - 38
IP  - 3
DP  - 1992 Sep
TI  - A two-site immunochemiluminometric assay for intact parathyroid hormone and its 
      clinical utility in hemodialysis patients.
PG  - 154-7
AB  - Measurement of serum parathyroid hormone (PTH) concentrations is complicated by 
      the fact that there are several fragments of the hormone in the circulating as a 
      result of the metabolism of PTH. The best way to study the secretory activity of 
      the parathyroid glands directly may be to measure the biologically active intact 
      PTH molecule. Recently, it has become possible to overcome these limitations by 
      application of the two-site immunoradiometric assay (IRMA) to the measurement of 
      intact PTH. The two-site immunometric technique has been applied to the two-site 
      immunochemiluminescent assay (ICMA) for the measurement of circulating intact 
      PTH. To evaluate the utility of measurements of serum intact PTH by two-site ICMA 
      in hemodialysis patients, two-site ICMA and IRMA were compared in 104 
      hemodialysis patients. We found a good correlation (r = 0.93) between values 
      obtained by ICMA and by IRMA. Although serum intact PTH by ICMA was significantly 
      suppressed by rising plasma ionized calcium after a session of hemodialysis in 29 
      patients, mid-region PTH did not show a significant change. This result suggests 
      the intact PTH assay is more suitable for the earlier detection of secondary 
      hyperparathyroidism than the mid-region assay and may be useful for precise 
      assessment of clinical status and response to therapeutic intervention designed 
      to correct the progressive bone disease in hemodialysis patients. We conclude 
      that two-site ICMA for serum intact PTH which could be measured without 
      radioisotopes nor scintillation counter is as useful as IRMA in hemodialysis 
      patients.
FAU - Morita, A
AU  - Morita A
AD  - Inoue Hospital, Suita, Japan.
FAU - Tabata, T
AU  - Tabata T
FAU - Koyama, H
AU  - Koyama H
FAU - Emoto, M
AU  - Emoto M
FAU - Inoue, T
AU  - Inoue T
FAU - Miki, T
AU  - Miki T
FAU - Nishizawa, Y
AU  - Nishizawa Y
FAU - Morii, H
AU  - Morii H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Clin Nephrol
JT  - Clinical nephrology
JID - 0364441
RN  - 0 (Parathyroid Hormone)
SB  - IM
MH  - Humans
MH  - Hyperparathyroidism, Secondary/blood/*diagnosis
MH  - Immunoradiometric Assay/methods
MH  - Luminescent Measurements
MH  - Middle Aged
MH  - Parathyroid Hormone/*blood
MH  - *Renal Dialysis
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
PST - ppublish
SO  - Clin Nephrol. 1992 Sep;38(3):154-7.