PMID- 1395732 OWN - NLM STAT- MEDLINE DCOM- 19921113 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 61 IP - 2 DP - 1992 TI - Detection of cell-cycle stage by fluorescence in situ hybridization: its application in human interphase cytogenetics. PG - 91-4 AB - Distinct cell-cycle-dependent changes in the conformation of centromeric chromatin in a specific human chromosome containing alpha-satellite DNA have been demonstrated by fluorescence in situ hybridization (FISH). This method, based upon specific FISH signal morphology, allows simultaneous analysis of chromosomal aneuploidy and detection of specific cell-cycle stage(s) of human tumor and/or normal cell populations in a single preparation of interphase cells. This interphase cytogenetic procedure might prove useful for both basic and clinical research involving human cells. FAU - Mukherjee, A B AU - Mukherjee AB AD - Laboratory of Cancer Genetics, Sloan-Kettering Institute, New York, NY. FAU - Murty, V V AU - Murty VV FAU - Chaganti, R S AU - Chaganti RS LA - eng GR - CA05826/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 SB - IM MH - *Cell Cycle MH - Cell Line MH - Centromere MH - *Chromosomes, Human, Pair 12 MH - Cytogenetics/methods MH - Humans MH - In Situ Hybridization MH - *Interphase MH - Karyotyping MH - Kinetics MH - Male MH - Spectrometry, Fluorescence MH - Tumor Cells, Cultured EDAT- 1992/01/01 00:00 MHDA- 1992/01/01 00:01 CRDT- 1992/01/01 00:00 PHST- 1992/01/01 00:00 [pubmed] PHST- 1992/01/01 00:01 [medline] PHST- 1992/01/01 00:00 [entrez] AID - 10.1159/000133378 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1992;61(2):91-4. doi: 10.1159/000133378.