PMID- 1400001
OWN - NLM
STAT- MEDLINE
DCOM- 19921112
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 73
IP  - 2
DP  - 1992 Aug
TI  - Acute and long-term TNF-alpha administration increases pulmonary vascular 
      reactivity in isolated rat lungs.
PG  - 708-12
AB  - Tumor necrosis factor-alpha (TNF-alpha) causes pulmonary hypertension and 
      arterial hypoxemia, but the mechanisms are unknown. We conducted two experiments 
      to test the hypothesis that TNF-alpha alters pulmonary vascular reactivity, which 
      in turn could cause either pulmonary hypertension or arterial hypoxemia. In 
      experiment 1, rats were given acute or long-term injections of TNF-alpha 
      (recombinant human) in vivo. Rats treated acutely received either saline or 
      TNF-alpha (40 micrograms/kg iv in saline) 3 min (TNF-3 min; n = 8), 20 min 
      (TNF-20 min; n = 8), or 24 h (TNF-24 h; n = 5) before the lungs were isolated. 
      Rats treated chronically received injections of either saline or TNF-alpha (250 
      micrograms/kg ip in saline) two times per day for 7 days (TNF-7 days; n = 9). 
      Lungs were isolated and perfused with Earle's salt solution (+2 g/l NaHCO3 + 4 
      g/100 ml Ficoll), and vascular reactivity was tested with acute hypoxia (3 min; 
      3% O2) and angiotensin II (ANG II; 0.025-0.40 micrograms). Pulmonary pressor 
      responses to hypoxia were greater (P less than 0.05) in TNF-20 min and TNF-7 day 
      groups. ANG II responses were increased (P less than 0.05) in TNF-7 day rats. In 
      experiment 2, lungs were isolated and perfused and received direct pulmonary 
      arterial injections of TNF-alpha (0.2, 2.0, and 20 micrograms) or saline, after 
      stable responses to hypoxia and ANG II (0.10 microgram) were attained. Reactivity 
      was not different between control and TNF-alpha rats before the injections, but 
      TNF-alpha increased (P less than 0.05) responses to hypoxia and ANG II.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Stevens, T
AU  - Stevens T
AD  - Department of Physiology, Colorado State University, Fort Collins 80523.
FAU - Janssen, P L
AU  - Janssen PL
FAU - Tucker, A
AU  - Tucker A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/pharmacology
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Female
MH  - Hematocrit
MH  - In Vitro Techniques
MH  - Lung/*drug effects
MH  - Organ Size/drug effects
MH  - Pulmonary Circulation/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 10.1152/jappl.1992.73.2.708 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1992 Aug;73(2):708-12. doi: 10.1152/jappl.1992.73.2.708.