PMID- 1405603
OWN - NLM
STAT- MEDLINE
DCOM- 19921029
LR  - 20190817
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 53
IP  - 2
DP  - 1992 Aug
TI  - Divergent effects of TNF alpha in the adoptive immunotherapy of a murine sarcoma.
PG  - 152-7
AB  - We have previously described an in vitro sensitization (IVS) procedure which 
      enabled the generation of therapeutic T cells from tumor-bearing mice for 
      adoptive immunotherapy. The procedure involved culture of tumor-draining lymph 
      node (TDLN) cells with irradiated tumor in the presence of interleukin-2 (IL-2). 
      The availability of many recombinant cytokines affords an opportunity to examine 
      their effects on the immune response to tumor. In this study, we investigated the 
      effect of tumor necrosis factor-alpha (TNF alpha) on the generation and function 
      of IVS cells utilized in adoptive immunotherapy of the murine MCA 106 sarcoma. 
      TNF alpha administered iv at nontherapeutic doses was found to enhance the 
      antitumor efficacy mediated by IVS cells plus IL-2 in the treatment of pulmonary 
      metastases. In contrast, TNF alpha administration to mice bearing progressive 
      footpad tumors had inhibitory effects on the sensitization of tumor-reactive 
      cells in TDLN since IVS cells generated from these animals displayed a diminished 
      antitumor effect. This effect appeared to be due to a reduced number of 
      tumor-reactive lymphoid cells in the TDLN since TNF alpha added to IVS cultures 
      did not alter the antitumor efficacy of the resultant IVS effector cells. These 
      findings indicate the divergent effects of TNF alpha on the immune response to 
      tumor and adoptive immunotherapy with IVS cells.
FAU - Wagner, P D
AU  - Wagner PD
AD  - Division of Surgical Oncology, University of Michigan, Ann Arbor 48109.
FAU - Shu, S
AU  - Shu S
FAU - Chang, A E
AU  - Chang AE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Female
MH  - *Immunotherapy, Adoptive/methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rats
MH  - Sarcoma, Experimental/*therapy
MH  - Tumor Necrosis Factor-alpha/administration & dosage/*therapeutic use
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0022-4804(92)90027-W [pii]
AID - 10.1016/0022-4804(92)90027-w [doi]
PST - ppublish
SO  - J Surg Res. 1992 Aug;53(2):152-7. doi: 10.1016/0022-4804(92)90027-w.