PMID- 1406687
OWN - NLM
STAT- MEDLINE
DCOM- 19921116
LR  - 20211203
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 12
IP  - 11
DP  - 1992 Nov
TI  - Tumorigenicity of the met proto-oncogene and the gene for hepatocyte growth 
      factor.
PG  - 5152-8
AB  - The met proto-oncogene is the tyrosine kinase growth factor receptor for 
      hepatocyte growth factor/scatter factor (HGF/SF). It was previously shown that, 
      like the oncogenic tpr-met, the mouse met proto-oncogene transforms NIH 3T3 
      cells. We have established NIH 3T3 cells stably expressing both human (Methu) and 
      mouse (Metmu) met proto-oncogene products. The protein products are properly 
      processed and appear on the cell surface. NIH 3T3 cells express endogenous mouse 
      HGF/SF mRNA, suggesting an autocrine activation mechanism for transformation by 
      Metmu. However, the tumor-forming activity of Methu in NIH 3T3 cells is very low 
      compared with that of Metmu, but efficient tumorigenesis occurs when Methu and 
      HGF/SFhu are coexpressed. These results are consistent with an autocrine 
      transformation mechanism and suggest further that the endogenous murine factor 
      inefficiently activates the tumorigenic potential of Methu. The tumorigenicity 
      observed with reciprocal chimeric human and mouse receptors that exchange 
      external ligand-binding domains supports this conclusion. We also show that 
      HGF/SFhu expressed in NIH 3T3 cells produces tumors in nude mice.
FAU - Rong, S
AU  - Rong S
AD  - ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, 
      Maryland 21702.
FAU - Bodescot, M
AU  - Bodescot M
FAU - Blair, D
AU  - Blair D
FAU - Dunn, J
AU  - Dunn J
FAU - Nakamura, T
AU  - Nakamura T
FAU - Mizuno, K
AU  - Mizuno K
FAU - Park, M
AU  - Park M
FAU - Chan, A
AU  - Chan A
FAU - Aaronson, S
AU  - Aaronson S
FAU - Vande Woude, G F
AU  - Vande Woude GF
LA  - eng
GR  - N01-CO-74101/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Gene Expression Regulation
MH  - Hepatocyte Growth Factor/genetics/*physiology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Neoplasms, Experimental/genetics
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins/genetics/*physiology
MH  - Proto-Oncogene Proteins c-met
MH  - *Proto-Oncogenes
PMC - PMC360449
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
PMCR- 1992/11/01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PHST- 1992/11/01 00:00 [pmc-release]
AID - 10.1128/mcb.12.11.5152-5158.1992 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1992 Nov;12(11):5152-8. doi: 10.1128/mcb.12.11.5152-5158.1992.