PMID- 14079487
OWN - NLM
STAT- MEDLINE
DCOM- 19961201
LR  - 20190509
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 18
IP  - 2
DP  - 1963 Aug
TI  - DYNAMICS OF ACRIDINE ORANGE-CELL INTERACTION. I. INTERRELATIONSHIPS OF ACRIDINE 
      ORANGE PARTICLES AND CYTOPLASMIC REDDENING.
PG  - 237-50
AB  - The in vitro localization of acridine orange (AO) in living cells was monitored 
      by means of fluorescence microscopy, quantitative cell viability studies, and 
      photofluorimetric measurements following dye-cell interaction. The parameters, 
      pH, time, dye concentration, and the metabolic state of the cell were found to 
      exert a profound influence on the time course and distribution of staining. The 
      parameters studied are mutually interdependent, and intracellular dye 
      localization may be predictably altered by their appropriate manipulation. 
      Conditions are defined whereby two morphologically distinct but physiologically 
      interrelated reactions, namely, acridine orange particle (AOP) formation and 
      cytoplasmic reddening (CR) may be caused, prevented, reversed, or modified. These 
      results are explained in terms of the facilitation or inhibition of an 
      intracytoplasmic dye-segregating mechanism, in turn affected by the rate of dye 
      ingress and the physiological state of the cell. Whereas the accumulation of AO 
      in AOP is compatible with cell viability, the appearance of CR is correlated with 
      cell death. It is pointed out that meaningful interpretation of vital staining 
      requires precise regulation of many parameters in the extracellular milieu. A 
      scheme of cell compartmentalization with respect to AO is proposed to 
      satisfactorily account for the effects of environmental variations on the 
      distribution and ultimate fate of intracellular dye. The AOP are viewed as 
      normally present acid phosphatase-positive multivesicular bodies.
FAU - ROBBINS, E
AU  - ROBBINS E
FAU - MARCUS, P I
AU  - MARCUS PI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Acridines)
RN  - 0 (Coloring Agents)
RN  - F30N4O6XVV (Acridine Orange)
SB  - OM
MH  - *Acridine Orange
MH  - *Acridines
MH  - *Cell Biology
MH  - *Cell Communication
MH  - *Cell Nucleus
MH  - *Coloring Agents
MH  - *Cytoplasm
MH  - *Fluorescence
MH  - *Histocytochemistry
MH  - *Hydrogen-Ion Concentration
MH  - *Metabolism
MH  - *Microscopy
MH  - *Microscopy, Fluorescence
MH  - *Organelles
MH  - *Staining and Labeling
PMC - PMC2106306
OTO - NLM
OT  - *ACRIDINES
OT  - *CELL NUCLEUS
OT  - *CYTOLOGY
OT  - *HISTOCYTOCHEMISTRY
OT  - *HYDROGEN-ION CONCENTRATION
OT  - *METABOLISM
OT  - *MICROSCOPY, FLUORESCENCE
OT  - *PROTOPLASM
OT  - *STAINS AND STAINING
EDAT- 1963/08/01 00:00
MHDA- 1963/08/01 00:01
PMCR- 1964/02/01
CRDT- 1963/08/01 00:00
PHST- 1963/08/01 00:00 [pubmed]
PHST- 1963/08/01 00:01 [medline]
PHST- 1963/08/01 00:00 [entrez]
PHST- 1964/02/01 00:00 [pmc-release]
AID - 10.1083/jcb.18.2.237 [doi]
PST - ppublish
SO  - J Cell Biol. 1963 Aug;18(2):237-50. doi: 10.1083/jcb.18.2.237.