PMID- 1409719
OWN - NLM
STAT- MEDLINE
DCOM- 19921117
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 20
DP  - 1992 Oct 15
TI  - Brain-derived neurotrophic factor and neurotrophin 3 mRNAs in the peripheral 
      target fields of developing inner ear ganglia.
PG  - 9915-9
AB  - In situ hybridization was used to study the site and timing of the expression of 
      nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 
      3 (NT-3), and neurotrophin 5 (NT-5) mRNAs in the developing inner ear of the rat. 
      In the sensory epithelia, the levels of NGF and NT-5 mRNAs were below the 
      detection limit. NT-3 and BDNF mRNAs were expressed in the otic vesicle in 
      overlapping but also in distinct regions. Later in development, NT-3 transcripts 
      were localized to the differentiating sensory and supporting cells of the 
      auditory organ and vestibular maculae. In these sensory epithelia, the intensity 
      of NT-3 mRNA expression decreased in parallel with maturation. The expression of 
      BDNF mRNA was restricted to the sensory cells of both the auditory and vestibular 
      organs, including ampullary cristae. In bioassays, BDNF and NT-3, but not NGF, at 
      physiological concentrations induced neurite outgrowth from the statoacoustic 
      ganglion explants. These results demonstrate that NT-3 and BDNF, rather than NGF 
      and NT-5, are the primary neurotrophins present in the target fields of the 
      cochlear and vestibular neurons. Expression of NT-3 and BDNF mRNAs in the otic 
      vesicle before and during the ingrowth of neurites from the statoacoustic 
      ganglion suggests that NT-3 or BDNF or both may serve as chemoattractants for the 
      early nerve fibers. The results also suggest that these neurotrophins have a role 
      in later development of the cochlear and vestibular neurons.
FAU - Pirvola, U
AU  - Pirvola U
AD  - Department of Pathology, University of Helsinki, Finland.
FAU - Ylikoski, J
AU  - Ylikoski J
FAU - Palgi, J
AU  - Palgi J
FAU - Lehtonen, E
AU  - Lehtonen E
FAU - Arumae, U
AU  - Arumae U
FAU - Saarma, M
AU  - Saarma M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Culture Techniques
MH  - Ear, Inner/*embryology
MH  - Ganglia/embryology
MH  - Gene Expression
MH  - In Situ Hybridization
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurotrophin 3
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC50244
EDAT- 1992/10/15 00:00
MHDA- 1992/10/15 00:01
PMCR- 1993/04/15
CRDT- 1992/10/15 00:00
PHST- 1992/10/15 00:00 [pubmed]
PHST- 1992/10/15 00:01 [medline]
PHST- 1992/10/15 00:00 [entrez]
PHST- 1993/04/15 00:00 [pmc-release]
AID - 10.1073/pnas.89.20.9915 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9915-9. doi: 
      10.1073/pnas.89.20.9915.