PMID- 1412206
OWN - NLM
STAT- MEDLINE
DCOM- 19921119
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 66
IP  - 4
DP  - 1992 May 15
TI  - Purification of factor XIa inhibitor from human platelets.
PG  - 397-408
AB  - An inhibitor of activated factor XI (FXIa) in human platelets was recently 
      identified as an amyloid beta-protein precursor (APP). We purified an FXIa 
      inhibitor (XIaI) from the supernatant of activated human platelets, and assessed 
      its inhibitor activity toward FXIa amidolytic activity. Approximately 90 
      micrograms of XIaI that cross-reacted with anti-APP antibody was obtained from 
      two hundred units of platelet suspension by employing a six-step column 
      chromatography procedure. The molecular weight of the purified XIaI was 94,000. 
      The Ki value of XIaI to factor XIa was 526 +/- 120 pM, and the inhibition was 
      enhanced by the addition of ZnCl2. The amino-terminal sequence of XIaI was 
      L-E-V-P-T-D-G-N-A-, which is identical to that for the leucine (N18) to alanine 
      (N26) sequence of APP751 and the amino-terminal sequence of protease nexin-2.
FAU - Komiyama, Y
AU  - Komiyama Y
AD  - Department of Clinico-laboratory Medicine, Kansai Medical University, Osaka, 
      Japan.
FAU - Murakami, T
AU  - Murakami T
FAU - Egawa, H
AU  - Egawa H
FAU - Okubo, S
AU  - Okubo S
FAU - Yasunaga, K
AU  - Yasunaga K
FAU - Murata, K
AU  - Murata K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Chromogenic Compounds)
RN  - 0 (Oligopeptides)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 72194-57-1 (S 2366)
RN  - EC 3.4.21.27 (Factor XIa)
RN  - SZB83O1W42 (Pyrrolidonecarboxylic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amyloid beta-Protein Precursor/*isolation & purification/pharmacology
MH  - *Blood Coagulation
MH  - Blood Platelets/*chemistry
MH  - Chromatography, Affinity
MH  - Chromatography, Gel
MH  - Chromogenic Compounds
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Factor XIa/*antagonists & inhibitors
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligopeptides
MH  - Pyrrolidonecarboxylic Acid/analogs & derivatives
MH  - Sequence Homology
MH  - Serine Proteinase Inhibitors/*isolation & purification/pharmacology
EDAT- 1992/05/15 00:00
MHDA- 1992/05/15 00:01
CRDT- 1992/05/15 00:00
PHST- 1992/05/15 00:00 [pubmed]
PHST- 1992/05/15 00:01 [medline]
PHST- 1992/05/15 00:00 [entrez]
AID - 10.1016/0049-3848(92)90289-m [doi]
PST - ppublish
SO  - Thromb Res. 1992 May 15;66(4):397-408. doi: 10.1016/0049-3848(92)90289-m.