PMID- 1415569
OWN - NLM
STAT- MEDLINE
DCOM- 19921029
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 263
IP  - 3 Pt 2
DP  - 1992 Sep
TI  - Angiotensin II receptor subtypes in cultured rat renal mesangial cells.
PG  - F411-6
AB  - The selective angiotensin (ANG II) antagonists losartan (DuP 753) and PD 123319 
      have been shown to bind selectively to AT1 and AT2 subtypes, respectively. To 
      characterize ANG II receptor subtypes in mesangial cells, washed membranes were 
      incubated with 0.1 to 0.5 nM 125I-ANG II and increasing concentrations of 
      competitors. The inhibition of 125I-ANG II binding by losartan and PD 123319 was 
      biphasic, and LIGAND curve-fitting analysis revealed two populations of specific 
      binding sites. One subpopulation comprised 86% of the total and showed high 
      affinity for ANG II and losartan, but low affinity for the AT2 antagonists PD 
      123319 and CGP42112A, and thus appear identical to the recently cloned AT1 
      subtype. The remaining 14% of the sites showed nearly 100-fold lower affinity for 
      losartan and 10,000-fold higher affinity for PD 123319 relative to AT1 sites. 
      However, another AT2-selective antagonist, CGP42112A, showed little affinity for 
      these sites. Both classes of binding sites were inhibited by guanosine 
      5'-O-(3-thiophosphate) and pertussis toxin treatment. We propose that there are 
      two distinct G protein-coupled ANG II receptor subtypes (AT1A and AT1B) present 
      in renal mesangial cells.
FAU - Ernsberger, P
AU  - Ernsberger P
AD  - Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio 
      44106.
FAU - Zhou, J
AU  - Zhou J
FAU - Damon, T H
AU  - Damon TH
FAU - Douglas, J G
AU  - Douglas JG
LA  - eng
GR  - HL-22990/HL/NHLBI NIH HHS/United States
GR  - HL-41618/HL/NHLBI NIH HHS/United States
GR  - HL-44514/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Angiotensin Receptor Antagonists)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Tetrazoles)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 11128-99-7 (Angiotensin II)
RN  - 130663-39-7 (PD 123319)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - JMS50MPO89 (Losartan)
SB  - IM
MH  - Angiotensin II/antagonists & inhibitors/metabolism
MH  - Angiotensin Receptor Antagonists
MH  - Animals
MH  - Binding Sites/drug effects
MH  - Biphenyl Compounds/pharmacology
MH  - Cells, Cultured
MH  - GTP-Binding Proteins/antagonists & inhibitors
MH  - Glomerular Mesangium/cytology/*metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Imidazoles/pharmacology
MH  - Losartan
MH  - Pertussis Toxin
MH  - Pyridines/pharmacology
MH  - Rats
MH  - Receptors, Angiotensin/*metabolism
MH  - Tetrazoles/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 10.1152/ajprenal.1992.263.3.F411 [doi]
PST - ppublish
SO  - Am J Physiol. 1992 Sep;263(3 Pt 2):F411-6. doi: 10.1152/ajprenal.1992.263.3.F411.