PMID- 1421094 OWN - NLM STAT- MEDLINE DCOM- 19921223 LR - 20190718 IS - 0959-4965 (Print) IS - 0959-4965 (Linking) VI - 3 IP - 10 DP - 1992 Oct TI - Neurotrophin-induced trk receptor phosphorylation and cholinergic neuron response in primary cultures of embryonic rat brain neurons. PG - 885-8 AB - Tyrosine phosphorylation of trk type neurotrophin receptors in primary cultures of embryonic rat brain cells was studied by immunoprecipitation and immunoblotting. In cultures containing basal forebrain cholinergic neurons, but not in cultures of cerebral cortex, nerve growth factor (NGF) treatment for 4 min induced tyrosine phosphorylation of trk family proteins. Stimulation with brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3), resulted in a very robust phosphorylation signal in basal forebrain and cortical cultures, suggesting actions of these neurotrophins not only on cholinergic cells but probably on most embryonic brain neurons. Trk tyrosine phosphorylation was completely abolished by 5 microM K-252b. Inhibition was rapid, being evident by 30 s following addition of the drug. Corresponding stimulatory and inhibitory effects were seen for phospholipase-C gamma 1 (PLC gamma 1) and extracellular signal-regulated kinase 1 (Erk1), two enzymes involved in second messenger mechanisms. Our findings indicate involvement of trk receptor activation in the NGF response of basal forebrain cholinergic cells and provide evidence for widespread presence of BDNF and NT-3 responsive neurons in the embryonic brain. FAU - Knusel, B AU - Knusel B AD - Andrus Gerontology Center, University of Southern California, Los Angeles 90089. FAU - Rabin, S AU - Rabin S FAU - Widmer, H R AU - Widmer HR FAU - Hefti, F AU - Hefti F FAU - Kaplan, D R AU - Kaplan DR LA - eng GR - AG14080/AG/NIA NIH HHS/United States GR - N01-CO-74101/CO/NCI NIH HHS/United States GR - NS22933/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - Neuroreport JT - Neuroreport JID - 9100935 RN - 0 (Nerve Growth Factors) RN - 0 (Receptors, Cholinergic) RN - 0 (Recombinant Proteins) RN - 42HK56048U (Tyrosine) RN - EC 2.3.1.6 (Choline O-Acetyltransferase) SB - IM MH - Animals MH - Blotting, Western MH - Cells, Cultured MH - Choline O-Acetyltransferase/metabolism MH - Cholinergic Fibers/*physiology MH - Female MH - Humans MH - Nerve Growth Factors/*pharmacology MH - PC12 Cells MH - Phosphorylation MH - Pregnancy MH - Rats MH - Rats, Wistar MH - Receptors, Cholinergic/*metabolism MH - Recombinant Proteins/pharmacology MH - Tyrosine/metabolism EDAT- 1992/10/01 00:00 MHDA- 1992/10/01 00:01 CRDT- 1992/10/01 00:00 PHST- 1992/10/01 00:00 [pubmed] PHST- 1992/10/01 00:01 [medline] PHST- 1992/10/01 00:00 [entrez] AID - 10.1097/00001756-199210000-00016 [doi] PST - ppublish SO - Neuroreport. 1992 Oct;3(10):885-8. doi: 10.1097/00001756-199210000-00016.