PMID- 1423229
OWN - NLM
STAT- MEDLINE
DCOM- 19921222
LR  - 20190815
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 63
IP  - 1
DP  - 1992 Oct 1
TI  - Application of fluorescence in situ hybridization in hematological disorders.
PG  - 62-9
AB  - In the present study, chromosome changes in bone marrow (BM) or peripheral blood 
      (PB) cells from 13 patients with malignant hematologic disorders were analyzed by 
      classical cytogenetic techniques (G-banding) and fluorescence in situ 
      hybridization (FISH) procedures using centromere specific probes for chromosomes 
      1, 6, 7, 8, 9, 12, 18, 13/21, and X, and a DNA probe specific for the long arm of 
      chromosome Y. The cytogenetic data obtained with G-banding were in accord with 
      those obtained by FISH to metaphase chromosomes. Most significantly, FISH to 
      interphase nuclei offered reliable results and in some cases provided important 
      information concerning crucial chromosome anomalies which were not or could not 
      be completely detected by analyzing metaphase chromosomes. Our results indicate 
      that FISH could be clinically valuable in five major areas: 1) marker chromosome 
      identification; 2) identification of trisomy consistent with certain specific 
      hematological neoplasms; 3) clonal evaluation post observation of a single cell 
      with trisomy; 4) clonal evaluation post-sex-mismatched bone marrow 
      transplantation (BMT); and 5) residual disease detection following clinical 
      remission.
FAU - Chen, Z
AU  - Chen Z
AD  - Cancer Center of Southwest Biomedical Research Institute and Genetrix, Inc., 
      Scottsdale, Arizona 85251.
FAU - Morgan, R
AU  - Morgan R
FAU - Berger, C S
AU  - Berger CS
FAU - Sandberg, A A
AU  - Sandberg AA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor
MH  - Child, Preschool
MH  - DNA Probes
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - Sensitivity and Specificity
MH  - Severe Combined Immunodeficiency/*genetics
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 0165-4608(92)90066-H [pii]
AID - 10.1016/0165-4608(92)90066-h [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1992 Oct 1;63(1):62-9. doi: 10.1016/0165-4608(92)90066-h.