PMID- 14234778
OWN - NLM
STAT- MEDLINE
DCOM- 19961201
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 88
IP  - 5
DP  - 1964 Nov
TI  - ACRIDINE ORANGE BINDING BY MICROCOCCUS LYSODEIKTICUS.
PG  - 1249-56
AB  - Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange 
      binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 
      1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The 
      adsorption isotherm is consistent with a highly cooperative-type binding similar 
      to that observed with polyadenylic acid. The cells exhibit a strong buffering 
      action on the concentration of free AO which remains constant (1 mug/ml) over a 
      range from 5 to 95% saturation of the cells by AO. The cells stain either 
      fluorescent orange or green. The fraction stained orange is directly proportional 
      to the quantity of dye adsorbed, indicating that these cells bind a fixed amount 
      of AO (10% of dry weight). The green-stained cells contain less than 1% of the AO 
      bound to orange-stained cells. The results suggest that the abrupt increase in 
      amount of AO bound by the orange-stained cells occurs when the concentration of 
      free AO reaches a threshold concentration. Similar results were obtained with 
      Bacillus cereus. Mg increases the free AO concentration and the extent of binding 
      capacity of the cells.
FAU - BEERS, R F Jr
AU  - BEERS RF Jr
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Acridines)
RN  - 0 (Buffers)
RN  - 0 (Coloring Agents)
RN  - F30N4O6XVV (Acridine Orange)
SB  - OM
MH  - *Acridine Orange
MH  - *Acridines
MH  - Adsorption
MH  - *Bacillus cereus
MH  - *Bacteriological Techniques
MH  - Buffers
MH  - *Coloring Agents
MH  - *Fluorescence
MH  - *Micrococcus
MH  - *Micrococcus luteus
MH  - *Microscopy
MH  - *Microscopy, Fluorescence
MH  - *Research
MH  - *Staining and Labeling
PMC - PMC277401
OTO - NLM
OT  - *ACRIDINES
OT  - *ADSORPTION
OT  - *BACILLUS CEREUS
OT  - *BACTERIOLOGICAL TECHNICS
OT  - *BUFFERS
OT  - *EXPERIMENTAL LAB STUDY
OT  - *MICROCOCCUS
OT  - *MICROSCOPY, FLUORESCENCE
OT  - *STAINS AND STAINING
EDAT- 1964/11/01 00:00
MHDA- 1964/11/01 00:01
PMCR- 1964/11/01
CRDT- 1964/11/01 00:00
PHST- 1964/11/01 00:00 [pubmed]
PHST- 1964/11/01 00:01 [medline]
PHST- 1964/11/01 00:00 [entrez]
PHST- 1964/11/01 00:00 [pmc-release]
AID - 10.1128/jb.88.5.1249-1256.1964 [doi]
PST - ppublish
SO  - J Bacteriol. 1964 Nov;88(5):1249-56. doi: 10.1128/jb.88.5.1249-1256.1964.